Invitrogen

ATP1A1 Monoclonal Antibody (464.6)

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ATP1A1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ATP1A1 in HepG2 cells. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731). ATPA1 (Green). Nuclei (Blue) were counterstained using Hoechst 33258.

ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ATP1A1 in human enodmetrial glands within the uterus. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731). Note the absence of staining in the surrounding myometrial smooth muscle.

ATP1A1 Antibody in Western Blot (WB) — Western blot analysis of ATP1A1 in porcine proximal tubule protein. Sample was incubated in ATP1A1 monoclonal antibody (Product # MA1-16731).

ATP1A1 Antibody in Flow Cytometry (Flow) — Flow cytometry of ATP1A1 in A549 cells. Samples were incubated in ATP1A1 monoclonal antibody (Product # MA1-16731) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (Blue) and a matched isotype control (Orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.

ATP1A1 Antibody in Immunohistochemistry (IHC) — Published figure using ATP1A1 monoclonal antibody (Product # MA1-16731) in Immunohistochemistry

Product Details

MA1-16731

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:10,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:1,000	View 2 publications 2 publications
Flow Cytometry (Flow)	1:50-1:200	-
Immunoprecipitation (IP)	Assay-Dependent	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Bovine, Dog, Fruit fly, Guinea pig, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast
Published species	Amphibian, Guinea pig, Rat, Rhesus monkey
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	464.6
Immunogen	Purified Na,K-ATPase from rabbit renal outer medulla.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2060993

Product Specific Information

Do not boil samples prior to Western Blot.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pluripotent stem cell-derived corneal endothelial cells as an alternative to donor corneal endothelium in keratoplasty.

Stem cell reports

Ali M,Khan SY,Gottsch JD,Hutchinson EK,Khan A,Riazuddin SA

MA1-16731 was used in Immunohistochemistry to evaluate the efficacy of cryopreserved human embryonic stem cell (hESC)-derived corneal endothelial cells (CECs) to form a functional monolayer of corneal endothelium (CE) in rabbits and monkeys.

Tue Sep 14 00:00:00 EDT 2021

Changes in cellular Ca²⁺ and Na⁺ regulation during the progression towards heart failure in the guinea pig.

The Journal of physiology

Ke HY,Yang HY,Francis AJ,Collins TP,Surendran H,Alvarez-Laviada A,Firth JM,MacLeod KT

MA1-16731 was used in Immunocytochemistry-immunoflourescence to follow changes in cardiac myocyte Ca2+ and Na+ regulation until signs of heart failure.

Wed Apr 01 00:00:00 EDT 2020

Changes in cellular Ca²⁺ and Na⁺ regulation during the progression towards heart failure in the guinea pig.

The Journal of physiology

Ke HY,Yang HY,Francis AJ,Collins TP,Surendran H,Alvarez-Laviada A,Firth JM,MacLeod KT

MA1-16731 was used in Immunocytochemistry-immunoflourescence to follow changes in cardiac myocyte Ca2+ and Na+ regulation until signs of heart failure.

Wed Apr 01 00:00:00 EDT 2020

Changes in cellular Ca²⁺ and Na⁺ regulation during the progression towards heart failure in the guinea pig.

The Journal of physiology

Ke HY,Yang HY,Francis AJ,Collins TP,Surendran H,Alvarez-Laviada A,Firth JM,MacLeod KT

MA1-16731 was used in Immunocytochemistry-immunoflourescence to follow changes in cardiac myocyte Ca2+ and Na+ regulation until signs of heart failure.

Wed Apr 01 00:00:00 EDT 2020

Changes in cellular Ca²⁺ and Na⁺ regulation during the progression towards heart failure in the guinea pig.

The Journal of physiology

Ke HY,Yang HY,Francis AJ,Collins TP,Surendran H,Alvarez-Laviada A,Firth JM,MacLeod KT

MA1-16731 was used in Immunocytochemistry-immunoflourescence to follow changes in cardiac myocyte Ca2+ and Na+ regulation until signs of heart failure.

Wed Apr 01 00:00:00 EDT 2020

A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes.

PloS one

Booth AE,Tarafder AK,Hume AN,Recchi C,Seabra MC

MA1-16731 was used in immunocytochemistry to analyze regulation of Rab27a localisation on melanosomes by Na+,K+-ATPase alpha1

Fri Dec 04 00:00:00 EST 2015

Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.

European journal of oral sciences

Wen X,Lacruz RS,Smith CE,Paine ML

MA1-16731 was used in immunohistochemistry and western blot to study the rat enamel cell expression of Na(+)/K(+)-ATPase subunits

Sat Feb 01 00:00:00 EST 2014

Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.

European journal of oral sciences

Wen X,Lacruz RS,Smith CE,Paine ML

MA1-16731 was used in immunohistochemistry and western blot to study the rat enamel cell expression of Na(+)/K(+)-ATPase subunits

Sat Feb 01 00:00:00 EST 2014

Altered Na+ transport after an intracellular alpha-subunit deletion reveals strict external sequential release of Na+ from the Na/K pump.

Proceedings of the National Academy of Sciences of the United States of America

Yaragatupalli S,Olivera JF,Gatto C,Artigas P

MA1-16731 was used in western blot to investigate the important role of the alpha-subunit in external release of sodium from the sodium/potassium pump

Tue Sep 08 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ -ATPase alpha 1 subunit; Na+/K+ ATPase; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na+K+ ATPase; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na/K ATPase alpha 1 subunit; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-ATPase alpha-subunit 1; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product

Gene Aliases: ATP1A1; atp1a1-a; atp1a1-b; atp1a1a.4; Atpa-1; BC010319; K-ATPase; Na+K+ATPase; Nkaa1b

UniProt ID: (Dog) P50997, (Sheep) P04074, (Rabbit) Q9N0Z6, (Human) P05023, (Pig) P05024, (Bovine) Q08DA1, (Rat) P06685, (Mouse) Q8VDN2

Entrez Gene ID: (Dog) 403992, (Sheep) 443381, (Rabbit) 100302415, (Human) 476, (Pig) 397481, (Bovine) 282144, (Guinea pig) 100192382, (Rat) 24211, (Mouse) 11928, (Western clawed frog) 395044