Invitrogen

ATP1A1 Monoclonal Antibody (9-A5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

10 References

View all (40) ATP1A1 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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ATP1A1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on human renal carcinoma tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

ATP1A1 Antibody — Fig. 12 Lubiprostone-induced redistribution of Na-K-ATPase, protein kinase A, and beta-catenin in rat proximal colon. Left saline treated tissue; right lubiprostone (0.25 ug) treated tissue. a Distribution of Na-K-ATPase ( white ) in surface, upper crypt ( orange dotted line ), and lower crypt ( yellow dotted line ) epithelia. Asterisks denote lumen of crypt base. b Distribution of protein kinase A (PKA) ( white ) and 5-HT-labeled enterochromaffin (EC) cells ( red ) in surface and crypt ( dotted line ) epithelia. High power inset 5-HT/PKA double labeled EC cell. c beta-Catenin labeled ( white ) surface and crypt ( dotted line ) epithelia. d Normalized Na-K-ATPase fluorescence intensity (FI) in lower crypt, upper crypt, and surface epithelia. Normalized PKA FI in lower crypt, upper crypt, and surface epithelia ( e ), and 5-HT-labeled EC cells ( f ). Asterisk significant difference from untreated group. Scale bars a 25 um; b - c , 100 um; inset, 5 um {TM}

Product Details

MA3-924

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1/100	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	View 1 publication 1 publication
Flow Cytometry (Flow)	1/50	-
ELISA (ELISA)	Assay-dependent	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	-
Inhibition Assays (IA)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Dog, Chicken, Human, Mouse, Rat
Published species	Human, Mouse, Pig, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	9-A5
Immunogen	Purified rat kidney sodium/potassium ATPase. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2061129

Product Specific Information

MA3-924 detects the alpha 1 sodium/potassium ATPase in human, mouse, canine, rat and avian tissues.

MA3-924 has been successfully used in immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA and immunoprecipitation procedures. Immunohistochemical staining of sodium/potassium ATPase in rat liver with MA3-924 yields a pattern consistent with plasma membrane localization. Immunoprecipitation of sodium/potassium ATPase from canine kidney extracts can be used for affinity purification of the alpha subunit. MA3-924 can also be utilized to inhibit activity of the enzyme.

The MA3-924 antigen is purified rat kidney sodium/potassium ATPase. This antibody recognizes an epitope within an intracellular region at or near Asp-369 of the sodium/potassium ATPase alpha subunit.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization of CFTR High Expresser cells in the intestine.

American journal of physiology. Gastrointestinal and liver physiology

Jakab RL,Collaco AM,Ameen NA

MA3-924 was used in immunohistochemistry to study the function of CFTR High Expresser cells in the intestine of rats

Sun Sep 15 00:00:00 EDT 2013

Lubiprostone targets prostanoid signaling and promotes ion transporter trafficking, mucus exocytosis, and contractility.

Digestive diseases and sciences

Jakab RL,Collaco AM,Ameen NA

Published figure using ATP1A1 monoclonal antibody (Product # MA3-924) in Immunofluorescence

Thu Nov 01 00:00:00 EDT 2012

The tetraspanin protein peripherin-2 forms a complex with melanoregulin, a putative membrane fusion regulator.

Biochemistry

Boesze-Battaglia K,Song H,Sokolov M,Lillo C,Pankoski-Walker L,Gretzula C,Gallagher B,Rachel RA,Jenkins NA,Copeland NG,Morris F,Jacob J,Yeagle P,Williams DS,Damek-Poprawa M

MA3-924 was used in immunocytochemistry to study the interaction between tetraspanin protein peripherin-2 and melanoregulin

Tue Feb 06 00:00:00 EST 2007

The tetraspanin protein peripherin-2 forms a complex with melanoregulin, a putative membrane fusion regulator.

Biochemistry

Boesze-Battaglia K,Song H,Sokolov M,Lillo C,Pankoski-Walker L,Gretzula C,Gallagher B,Rachel RA,Jenkins NA,Copeland NG,Morris F,Jacob J,Yeagle P,Williams DS,Damek-Poprawa M

MA3-924 was used in immunocytochemistry to study the interaction between tetraspanin protein peripherin-2 and melanoregulin

Tue Feb 06 00:00:00 EST 2007

Involvement of IL-10 in exhaustion of myeloid dendritic cells and rescue by CD40 stimulation.

Immunology

Kajino K,Nakamura I,Bamba H,Sawai T,Ogasawara K

MA3-924 was used in ELISA to study the role of interleukin 10 in the function of myeloid dendritic cells

Mon Jan 01 00:00:00 EST 2007

A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.

The Journal of biological chemistry

Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM

MA3-924 was used in immunohistochemistry to study the mechanism in CCR5 cell surface expression.

Fri Oct 26 00:00:00 EDT 2001

Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression.

The American journal of physiology

Simon FR,Leffert HL,Ellisman M,Iwahashi M,Deerinck T,Fortune J,Morales D,Dahl R,Sutherland E

MA3-924 was used in immunohistochemistry to investigate the relationship between hepatic sodium/potassium ATPase activity and the expression of polarized beta subunit

Sat Jul 01 00:00:00 EDT 1995

Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy.

FEBS letters

Ting-Beall HP,Beall HC,Hastings DF,Friedman ML,Ball WJ

MA3-924 was used in crystallagraphy to investigate the binding domains of sodium/potassium ATPase antibody.

Mon Jun 04 00:00:00 EDT 1990

Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping.

Biochemistry

Hubert JJ,Schenk DB,Skelly H,Leffert HL

MA3-924 was used in neutralization experiments in rat liver

Tue Jul 15 00:00:00 EDT 1986

Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.

The Journal of biological chemistry

Schenk DB,Hubert JJ,Leffert HL

MA3-924 was used in blocking/activating experiment to investigate the activity and sites of sodium/potassium ATPase in normal and regenerating rat liver

Mon Dec 10 00:00:00 EST 1984

Bioinformatics

Protein Aliases: (Na+ + K+)-ATPase; ATP1B; ATPase; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium-transporting ATPase subunit alpha-1

Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b

UniProt ID: (Dog) P50997, (Human) P05023, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2

Entrez Gene ID: (Dog) 403992, (Human) 476, (Chicken) 396530, (Rat) 24211, (Mouse) 11928

Molecular Function: primary active transporter