Invitrogen

ATP6V1B1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (19) V-ATPase B1 antibodies

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Protocols

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ATP6V1B1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of V-ATPase B1 was performed using 70% confluent log phase HaCaT and BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with V-ATPase B1 Polyclonal Antibody (Product # PA5-56878) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing endosomal localization. Panel e represents BeWo cells where the expression of the target protein is relatively low. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ATP6V1B1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of V-ATPase B1 in human kidney and liver tissues using V-ATPase B1 Polyclonal Antibody (Product # PA5-56878). Corresponding ATP6V1B1 RNA-seq data are presented for the same tissues.

ATP6V1B1 Antibody in Western Blot (WB) — Western blot was performed using Anti-V-ATPase B1 Polyclonal Antibody (Product # PA5-56878) and a 56 kDa band corresponding to V-ATPase B1 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of SK-BR-3 (Lane 1), HaCaT (Lane 2), HT-29 (Lane 3), BeWo (Lane 4), IMR-32 (Lane 5), Raji (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). A non-specific band picked up below 40 kDa marker has been marked with an asterisk.

ATP6V1B1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of V-ATPase B1 demonstrates antibody specificity. Immunohistochemical analysis of V-ATPase B1 using anti-V-ATPase B1 Polyclonal Antibody (Product # PA5-56878), shows significant staining of V-ATPase B1 in human kidney and shows minimal or weak staining in human liver tissues. The relative expression levels of V-ATPase B1 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-56878

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human V-ATPase B1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2649398

Product Specific Information

Immunogen sequence: MAMEIDSRPG GLPGSSCNLG AAREHMQAVT RNYITHPRVT YRTVCSVNGP LVVLDRVKFA QYAEIV

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 77%.

Target Information

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A new Elf5^Cre ^ERT ^2- ^GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues.

FEBS letters

Singh S,Elenio E,Leu NA,Romano RA,Vaughan AE,DeRiso J,Surendran K,Chakrabarti R

PA5-56878 was used in Immunocytochemistry-Immunofluorescence to show that Elf5+ cells are present in several adult tissues, where its expression was previously not known.

Wed May 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; Endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; H+-ATPase beta 1 subunit; V-ATPase B1; V-ATPase B1 subunit; V-ATPase subunit B 1; V-type proton ATPase subunit B, kidney isoform; vacuolar proton pump 3; Vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3

Gene Aliases: ATP6B1; ATP6V1B1; RTA1B; VATB; VMA2; VPP3

UniProt ID: (Human) P15313

Entrez Gene ID: (Human) 525

Molecular Function: ATP synthase

Function(s)

ossification regulation of pH excretion sensory perception of sound insulin receptor signaling pathway ATP hydrolysis coupled proton transport proton transport regulation of macroautophagy transferrin transport ion transmembrane transport inner ear morphogenesis pH reduction ATP metabolic process calcium ion homeostasis phagosome acidification

Process(es)

ATP binding hydrogen ion transmembrane transporter activity hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances protein complex binding

ATP6V1B1 Polyclonal Antibody

Product Details

PA5-56878

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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