OriGene

ATP6V1B2 Monoclonal Antibody (OTI1E11), TrueMAB™

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ATP6V1B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-ATP6V1B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505244)

ATP6V1B2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B2 (RC205447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP. Positive lysates LY419793 (100 µg) and LC419793 (20 µg) can be purchased separately from OriGene.

Product Details

TA505244

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI1E11
Immunogen	Full length human recombinant protein of human ATP6V1B2 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

ATP6V1B2 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2; Endomembrane proton pump 58 kDa subunit; H+ transporting two-sector ATPase; HO57; testicular secretory protein Li 65; V-ATPase B2 subunit; V-ATPase subunit B 2; V-type proton ATPase subunit B, brain isoform; vacuolar H+-ATPase 56,000 subunit; Vacuolar proton pump subunit B 2

Gene Aliases: ATP6B1B2; ATP6B2; ATP6V1B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2

UniProt ID: (Human) P21281

Entrez Gene ID: (Human) 526

Molecular Function: ATP synthase