Western blot analysis of ATP6V1E1 in lysate expressing protein V-type proton ATPase subunit E. Samples were probed with an ATP6V1E1 polyclonal antibody (Product # PA1-27800) at a dilution of 1:1000 dilution. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate. Gene specific transient over-expression HEK293T cell lysate (lane 2); Empty vector transfected control HEK293T cell lysate (lane 3).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to human Vacuolar ATP synthase subunit E.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27800 detects ATP6V1E1 from human, mouse, and rat samples.
PA1-27800 may be successfully used in Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The immunogen is a synthetic peptide corresponding to human Vacuolar ATP synthase subunit E.
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This protein is a component of vacuolar ATPase (V-ATP
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