OriGene

ATP6V1F Monoclonal Antibody (OTI6C11), TrueMAB™

Product Image Gallery

ATP6V1F Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6. Positive lysates LY418132 (100 µg) and LC418132 (20 µg) can be purchased separately from OriGene.

ATP6V1F Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC210728 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1F antibody (TA502322), and then analyzed by flow cytometry.

Product Details

TA502322

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:500	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Dog, Human, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI6C11
Immunogen	Full length human recombinant protein of human ATP6V1F produced in E.coli. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.44 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: adenosinetriphosphatase 14k chain; ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F; ATPase, H+ transporting, V1 subunit F; ATPase, vacuolar, 14 kD; H(+)-transporting two-sector ATPase, 14kD subunit; V-ATPase 14 kDa subunit; V-ATPase F subunit; V-ATPase subunit F; V-type proton ATPase subunit F; vacuolar ATP synthase subunit F; vacuolar proton pump F subunit; Vacuolar proton pump subunit F

Gene Aliases: ATP6S14; ATP6V1F; VATF; Vma7

UniProt ID: (Human) Q16864, (Rat) P50408

Entrez Gene ID: (Dog) 475199, (Human) 9296, (Rat) 116664

Molecular Function: ATP synthase