CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (A21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent (R37117) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (R37605) and ActinGreen™ 488 ReadyProbes® reagent (R37110) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (P36930). Individual images were acquired on the EVOS® FL Auto imaging system with a 10X objective (AMEP4681) and stitched to a panorama using the tile/stitch function.
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Non-human primate, Bovine, Mouse|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Equine chorionic gonadotropin alters luteal cell morphologic features related to progesterone synthesis.
A-21355 was used in immunohistochemistry (paraffin) to test if eCG alters the content of luteal cells and mitochondria related to hormone production.
|Rigoglio NN,Fátima LA,Hanassaka JY,Pinto GL,Machado AS,Gimenes LU,Baruselli PS,Rennó FP,Moura CE,Watanabe IS,Papa PC||Theriogenology (79:673)||2013|
|Non-human primate||Not Cited||
Arenavirus infection induces discrete cytosolic structures for RNA replication.
A-21355 was used in western blot to identify and study cytosolic structures involved in arenavirus replication and transcription.
|Baird NL,York J,Nunberg JH||Journal of virology (86:11301)||2012|
Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.
A-21355 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.
|Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A||DNA repair (10:639)||2011|