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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to 13 amino acids near the center of human ATR.|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunohistochemistry (IHC)||2 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes all three isoforms.
A suggested positive control is HepG2 cell lysate.
PA5-20016 can be used with blocking peptide PEP-0135.
The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
2310008J16Rik; 2810405N18Rik; ataxia telangiectasia and Rad3 related; ataxia telangiectasia and Rad3-related protein; ATR serine/threonine kinase; ATR, TEM8; FRAP-related protein-1; MEC1, mitosis entry checkpoint 1, homolog; serine/threonine-protein kinase ATR; tumor endothelial marker 8
ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1