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NeoBiotechnologies
ATRX/RAD54 (Alpha Thalassemia Mental Retardation) Recombinant Mouse Monoclonal Antibody (rATRX, 3446)
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ATRX/RAD54 (Alpha Thalassemia Mental Retardation) Antibody (546-MSM5-P1ABX) in IHC (P)
Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human prostate using ATRX, RAD54 (Alpha Thalassemia Mental Retardation) Recombinant Monoclonal Antibody (Product # 546-MSM5-P1ABX).
Product Details
546-MSM5-P1ABX
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
rATRX, 3446
Immunogen
Recombinant full-length human ATRX protein
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
ATRX encodes a protein that contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae); ATP-dependent helicase ATRX; DNA dependent ATPase and helicase; helicase 2; helicase 2, X-linked; mental retardation, X-linked 52; RP5-875J14.1; Transcriptional regulator ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Zinc finger helicase; Znf-HX
Gene Aliases: ATR2; ATRX; JMS; MRX52; MRXHF1; RAD54; RAD54L; SFM1; SHS; XH2; XNP; ZNF-HX
UniProt ID: (Human) P46100
Entrez Gene ID: (Human) 546
DNA repair
DNA methylation
DNA recombination
nucleosome assembly
DNA replication-independent nucleosome assembly
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
spermatogenesis
positive regulation of nuclear cell cycle DNA replication
covalent chromatin modification
DNA damage response, signal transduction by p53 class mediator
forebrain development
replication fork processing
positive regulation of telomere maintenance
DNA duplex unwinding
post-embryonic forelimb morphogenesis
multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
Sertoli cell development
protein localization to chromosome, telomeric region
seminiferous tubule development
cellular response to hydroxyurea
regulation of histone H3-K9 trimethylation
negative regulation of telomeric RNA transcription from RNA pol II promoter
positive regulation of telomeric RNA transcription from RNA pol II promoter
negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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