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AXIN1 Monoclonal Antibody (1C4E8)

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AXIN1 Antibody in Western Blot (WB) — Various lysates (A431, MCF-7, HeLa, HEK-293, HepG2, Jurkat, K-562) were subjected to SDS PAGE followed by western blot with (Product # 68093-1-IG150UL) (AXIN1 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

AXIN1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with (Product # 68093-1-IG150UL) (AXIN1 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

AXIN1 Antibody in Western Blot (WB) — HSC-T6 cells were subjected to SDS PAGE followed by western blot with (Product # 68093-1-IG150UL) (AXIN1 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

Product Details

68093-1-IG150UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:50,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1C4E8
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a disheveled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Axin-1; Axis inhibition protein 1; axis inhibitor 1; fused, mouse, homolog of; GSK-3beta interacting protein rAxin; hAxin; LA16c-314G4.3; MGC52315; Protein Fused; protein phosphatase 1, regulatory subunit 49; rAxin

Gene Aliases: AI316800; AXIN; AXIN1; Fu; fused; Kb; Ki; kinky; knobbly; PPP1R49

UniProt ID: (Human) O15169, (Rat) O70239, (Mouse) O35625

Entrez Gene ID: (Human) 8312, (Rat) 79257, (Mouse) 12005

Molecular Function: scaffold/adaptor protein

optic placode formation positive regulation of protein phosphorylation apoptotic process activation of JUN kinase activity determination of left/right symmetry cell death dorsal/ventral axis specification positive regulation of peptidyl-threonine phosphorylation Wnt signaling pathway forebrain anterior/posterior pattern specification Wnt-activated signaling pathway involved in forebrain neuron fate commitment negative regulation of Wnt signaling pathway olfactory placode formation cytoplasmic microtubule organization positive regulation of protein ubiquitination activation of protein kinase activity positive regulation of peptidyl-serine phosphorylation negative regulation of transcription elongation from RNA polymerase II promoter regulation of catenin import into nucleus proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of GTPase activity cellular protein complex assembly negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of transcription, DNA-templated positive regulation of JNK cascade embryonic eye morphogenesis axial mesoderm formation positive regulation of ubiquitin-protein transferase activity muscle cell development canonical Wnt signaling pathway embryonic skeletal joint morphogenesis canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation cellular response to organic cyclic compound negative regulation of canonical Wnt signaling pathway Wnt signaling pathway involved in somitogenesis positive regulation of canonical Wnt signaling pathway beta-catenin destruction complex assembly beta-catenin destruction complex disassembly positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process protein polyubiquitination in utero embryonic development regulation of protein phosphorylation nucleocytoplasmic transport sensory perception of sound adult walking behavior dorsal/ventral pattern formation hemoglobin metabolic process protein catabolic process positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of proteasomal ubiquitin-dependent protein catabolic process erythrocyte homeostasis post-anal tail morphogenesis termination of G-protein coupled receptor signaling pathway positive regulation of JUN kinase activity positive regulation of protein kinase activity axial mesoderm development neurological system process negative regulation of protein metabolic process protein homooligomerization genetic imprinting signal transduction multicellular organism development cell differentiation

Process(es)

signal transducer activity GTPase activator activity protein binding beta-catenin binding enzyme binding protein kinase binding receptor signaling complex scaffold activity ubiquitin protein ligase binding protein complex scaffold identical protein binding protein homodimerization activity SMAD binding armadillo repeat domain binding I-SMAD binding p53 binding receptor binding protein C-terminus binding protein domain specific binding protein self-association R-SMAD binding