Invitrogen

Acetyl-CoA Carboxylase Monoclonal Antibody (B.800.8)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 References

View all (33) Acetyl-CoA Carboxylase antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Acetyl-CoA Carboxylase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Acetyl-CoA Carboxylase in NIH/3T3 cells using an Acetyl-CoA Carboxylase monoclonal antibody (Product # MA5-15025) (red). DNA is labeled using a fluorescent blue dye.

Acetyl-CoA Carboxylase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Acetyl-CoA Carboxylase in paraffin-embedded human lung carcinoma using an Acetyl-CoA Carboxylase monoclonal antibody (Product # MA5-15025).

Acetyl-CoA Carboxylase Antibody in Western Blot (WB) — Knockdown of AcetylCoA Carboxylase was achieved by transfecting HeLa cells with AcetylCoA Carboxylase specific siRNAs (Silencer® select Product # s882, s883). Western blot analysis (Fig. a) was performed using whole cell extracts from the AcetylCoA Carboxylase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with AcetylCoA Carboxylase Monoclonal Antibody (B.800.8) (Product # MA5-15025, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to AcetylCoA Carboxylase.

Acetyl-CoA Carboxylase Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Acetyl-coA Carboxylase siRNA and reduction of signal was observed in Western Blot using Acetyl-coA Carboxylase Monoclonal Antibody (B.800.8) (Product # MA5-15025). {KD}

Product Details

MA5-15025

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:200-1:800	-

Product Specifications
Species Reactivity	Hamster, Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Monoclonal
Type	Antibody
Clone	B.800.8
Immunogen	Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase a1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	210 µg/mL
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	<0.02% sodium azide
Storage conditions	-20°C
RRID	AB_10989186

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

MA5-15025 was used in Western Blotting to investigate the antiobesity and antidiabetic effects of the dairy bacterium P. freudenreichii MJ2 isolated from raw milk using 3T3-L1 cells and high-fat diet (HFD)-induced obese mice.

Thu Jan 28 00:00:00 EST 2021

Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro.

PloS one

McKay TB,Hjortdal J,Priyadarsini S,Karamichos D

MA515025 was used in western blot to report that hypoxia alters collagen and matrix metalloproteinase expression in human patients with keratoconus

Thu Sep 07 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: acaca; ACC-alpha; ACC1; Acetyl-CoA carboxylase 1; Acetyl-CoA carboxylase 265; Acetyl-Coenzyme A carboxylase alpha; EC 6.3.4.14; EC 6.4.1.2

Gene Aliases: A530025K05Rik; ACAC; ACACA; ACACAD; ACC; ACC1; ACCA; Gm738; I79_006999

UniProt ID: (Human) Q13085, (Mouse) Q5SWU9, (Rat) P11497

Entrez Gene ID: (Human) 31, (Mouse) 107476, (Chinese hamster) 100762904, (Rat) 60581

Function(s)

tissue homeostasis acetyl-CoA metabolic process lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process metabolic process response to organic cyclic compound multicellular organismal protein metabolic process protein homotetramerization lipid homeostasis cellular response to prostaglandin E stimulus biotin metabolic process carnitine shuttle positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process malonyl-CoA biosynthetic process response to drug

Process(es)

acetyl-CoA carboxylase activity biotin carboxylase activity protein binding ATP binding metal ion binding nucleotide binding catalytic activity biotin binding ligase activity kinase binding

Acetyl-CoA Carboxylase Monoclonal Antibody (B.800.8)

Product Details

MA5-15025

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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