Immunofluorescent analysis of acetylated Histone H3 (Lys9, green) in HEK293T cells left untreated (top row) or treated with 5uM of the HDAC inhibitor, Scriptaid (bottom row), for 24 hours. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:50 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||C. elegans, Human, Mouse, Non-human primate, Rat, Yeast, Zebrafish|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 0.15M NaCl, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:50|
|Flow Cytometry (Flow)||1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:800|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other acetylated histones.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes.
MA5-11195 was used in immunocytochemistry to study how chromosomal changes contribute to cytomixis
|Mursalimov S,Permyakova N,Deineko E,Houben A,Demidov D||Frontiers in plant science (6:null)||2015|