Immunofluorescent analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) (Product# MA3-042) shows staining in Neuro-2a Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase (Product# MA3-042) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Cat, Guinea pig, Human, Mouse, Rabbit|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified human cerebellar acetylcholinesterase.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunoprecipitation (IP)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-042 detects acetylcholinesterase (AChE) from human, mouse, rabbit, guinea pig, bovine and cat tissues. This antibody does not cross react with rat or frog AChE, and does not detect butyrylcholinesterase (BChE).
MA3-042 has been successfully used in immunohistochemistry, immunoprecipitation and ELISA procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of AChE in human brain samples with MA3-042 results in staining of nerve fibers and terminals.
The MA3-042 antigen is purified human cerebellar acetylcholinesterase.
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Functional variability in butyrylcholinesterase activity regulates intrathecal cytokine and astroglial biomarker profiles in patients with Alzheimer's disease.
MA3-042 was used in ELISA to study the effect of Alzheimer's disease patient butyrylcholinesterase genotype on the profile of inflammatory cytokines and markers of astroglial function in CSF
|Darreh-Shori T,Vijayaraghavan S,Aeinehband S,Piehl F,Lindblom RP,Nilsson B,Ekdahl KN,Långström B,Almkvist O,Nordberg A||Neurobiology of aging (34:2465)||2013|
Lesions of cortical GABAergic interneurons and acetylcholine neurons in xeroderma pigmentosum group A.
MA3-042 was used in immunohistochemistry to study the status of cortical GABAergic interneurons and cholinergic neurons in xeroderma pigmentosum group A
|Hayashi M,Ohto T,Shioda K,Fukatsu R||Brain and development (34:287)||2012|
Decrease in acetylcholinergic neurons in the pedunculopontine tegmental nucleus in a patient with Prader-Willi syndrome.
MA3-042 was used in immunohistochemistry to study the selective deficiency of acetylcholine neurons in the pedunculopontine tegmental nucleus with Prader-Willi syndrome
|Hayashi M,Miyata R,Tanuma N||Neuropathology : official journal of the Japanese Society of Neuropathology (31:280)||2011|
Fetal fornix transection and gestation length in sheep.
MA3-042 was used in immunohistochemistry to study the developmental state of fetal hippocampus and its role in gestation
|McDonald TJ,Li C,Vincent SE,Nijland MJ||Experimental neurology (200:532)||2006|
Immunocytochemical demonstration of axonal and perikaryal acetylcholinesterase in human cerebral cortex.
MA3-042 was used in immunohistochemistry to investigate the expression of acetylcholinesterase in axons and perikarya in the human cerebral cortex
|Mesulam MM,Geula C,Cosgrove R,Mash D,Brimijoin S||Brain research (539:233)||1991|
Altered levels of acetylcholinesterase in Alzheimer plasma.
MA3-042 was used in immunoprecipitation to investigate the changes of acetylcholinesterase level in Alzheimer plasma
|García-Ayllón MS,Riba-Llena I,Serra-Basante C,Alom J,Boopathy R,Sáez-Valero J||PloS one (5:null)||2010|
Different cholinesterase inhibitor effects on CSF cholinesterases in Alzheimer patients.
MA3-042 was used in western blot to investigate the effect of cholinesterase inhibitors on the actvities and protein levels of cholinesterases
|Nordberg A,Darreh-Shori T,Peskind E,Soininen H,Mousavi M,Eagle G,Lane R||Current Alzheimer research (6:4)||2009|