HYB 190-01-02 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and eruthrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.
HYB 190-01-02 has been successfully used in ELISA and Western blotting techniques. Can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects. HYB 190-01-02 is well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann's reaction). In Western blotting and dot blotting HYB 190-01-02 reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE.
The HYB 190-01-02 immunogen is the C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the eruthrocyte enzyme. Coupled to PPD with glutaraldehyde.
The epitope of HYB 190-01-02 is C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE).
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Acetylcholinesterase (AChE) is an enzyme located in the postsynaptic membrane and in the muscle endplates,
where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular
mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acetylcholinesterase; acetylcholinesterase (Yt blood group); AChE; apoptosis-related acetylcholinesterase; EC 18.104.22.168; YT; Yt blood group
Gene Aliases: ACEE; ACHE; ARACHE; BOS_23015; N-ACHE; YT