Invitrogen

Acetylcholinesterase Monoclonal Antibody (HR2)

Product Image Gallery

Acetylcholinesterase Antibody — Figure 3 Immunodetection of AChE subunit variants in human plasma, liver, RBCs, CSF and brain (frontal cortex). Samples were immunoblotted with three anti-AChE antibodies, ( A ) the N-terminal N-19, which recognizes all variants; ( B ) the C-terminal ab31276, which recognizes only AChE-T subunits; and ( C ) the anti- AChE-R antibody directed at the unique C-terminus of AChE-R. Representative immunoblot with the N-19 antibody of individual G 1 +G 2 peak fractions separated by sucrose gradient centrifugation from CSF and brain are included (A, right panel).

Acetylcholinesterase Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) (Product # MA3-042) shows staining in Neuro-2a Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase (Product # MA3-042) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Acetylcholinesterase Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on normal deparaffinized human Cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Acetylcholinesterase (Product # MA3-042) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Frozen) (IHC (F))

1:50

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

Assay dependent

Published Applications

ELISA (ELISA)

See 1 publication below

Immunohistochemistry (IHC)

See 5 publications below

Immunoprecipitation (IP)

See 1 publication below

Western Blot (WB)

See 3 publications below

Immunocytochemistry (ICC)

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Cat, Guinea pig, Human, Mouse, Rabbit

Published species

Human, Non-human primate

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

HR2

Immunogen

Purified human cerebellar acetylcholinesterase.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_325478

Product Specific Information

MA3-042 detects acetylcholinesterase (AChE) from human, mouse, rabbit, guinea pig, bovine and cat tissues. This antibody does not cross react with rat or frog AChE, and does not detect butyrylcholinesterase (BChE).

MA3-042 has been successfully used in immunohistochemistry, immunoprecipitation and ELISA procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of AChE in human brain samples with MA3-042 results in staining of nerve fibers and terminals.

The MA3-042 antigen is purified human cerebellar acetylcholinesterase.

Target Information

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Acetylcholinesterase; acetylcholinesterase (Yt blood group); AChE; apoptosis-related acetylcholinesterase; EC 3.1.1.7; Yt blood group

Gene Aliases: ACEE; ACHE; ARACHE; BOS_23015; N-ACHE; YT

UniProt ID: (Human) P22303, (Bovine) P23795, (Cat) O62763, (Mouse) P21836

Entrez Gene ID: (Rabbit) 100009390, (Human) 43, (Bovine) 540446, (Cat) 493674, (Mouse) 11423, (Guinea pig) 100422792

Molecular Function: esterase hydrolase lipase