Invitrogen

Acetylcholinesterase Monoclonal Antibody (HR2)

9 Published Figures

18 References

View all (20) Acetylcholinesterase antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (9)

Acetylcholinesterase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

Acetylcholinesterase Antibody (MA3-042) in ICC/IF

Immunofluorescent analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) (Product # MA3-042) shows staining in Neuro-2a Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an... View More

Acetylcholinesterase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Acetylcholinesterase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Acetylcholinesterase Antibody in Immunohistochemistry (IHC)

Acetylcholinesterase Antibody in Western Blot, Immunoprecipitation (WB, IP)

Acetylcholinesterase Antibody in Western Blot (WB)

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Product Details

MA3-042

Applications

Tested Dilution

Publications

Western Blot (WB)

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (Frozen) (IHC (F))

1:50

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Cat, Guinea pig, Human, Mouse, Rabbit

Published species

Guinea pig, Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

HR2

Immunogen

Purified human cerebellar acetylcholinesterase.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325478

Product Specific Information

MA3-042 detects acetylcholinesterase (AChE) from human, mouse, rabbit, guinea pig, bovine and cat tissues. This antibody does not cross react with rat or frog AChE, and does not detect butyrylcholinesterase (BChE).

MA3-042 has been successfully used in immunohistochemistry, immunoprecipitation and ELISA procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of AChE in human brain samples with MA3-042 results in staining of nerve fibers and terminals.

The MA3-042 antigen is purified human cerebellar acetylcholinesterase.

Target Information

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The SMYD1 and skNAC transcription factors contribute to neurodegenerative diseases.

Brain, behavior, & immunity - health

Mayfield RD,Zhu L,Smith TA,Tiwari GR,Tucker HO

MA3-042 was used in Immunoprecipitation to report that comparative analysis of genes deregulated by SMYD1 or skNAC knockdown in differentiating C2C12 myoblasts identified transcripts characteristic of neurodegenerative diseases, including Alzheimer's, Parkinson's and Huntington's Diseases (AD, PD, and HD).

Tue Dec 01 00:00:00 UTC 2020

Adventitial Cell Atlas of wt (Wild Type) and ApoE (Apolipoprotein E)-Deficient Mice Defined by Single-Cell RNA Sequencing.

Arteriosclerosis, thrombosis, and vascular biology

Gu W,Ni Z,Tan YQ,Deng J,Zhang SJ,Lv ZC,Wang XJ,Chen T,Zhang Z,Hu Y,Jing ZC,Xu Q

MA3-042 was used in Immunohistochemistry-immunofluorescence to study the role of the cellular atlas of the heterogeneous adventitial cells, and the intracellular communication, in atherosclerosis.

Sat Jun 01 00:00:00 UTC 2019

Cross-talk between microglia and neurons regulates HIV latency.

PLoS pathogens

Alvarez-Carbonell D,Ye F,Ramanath N,Garcia-Mesa Y,Knapp PE,Hauser KF,Karn J

Published figure using Acetylcholinesterase monoclonal antibody (Product # MA3-042) in Immunofluorescence

Sun Dec 01 00:00:00 UTC 2019

Cross-talk between microglia and neurons regulates HIV latency.

PLoS pathogens

Alvarez-Carbonell D,Ye F,Ramanath N,Garcia-Mesa Y,Knapp PE,Hauser KF,Karn J

Published figure using Acetylcholinesterase monoclonal antibody (Product # MA3-042) in Immunofluorescence

Sun Dec 01 00:00:00 UTC 2019

Identification of Carboxylesterase, Butyrylcholinesterase, Acetylcholinesterase, Paraoxonase, and Albumin Pseudoesterase in Guinea Pig Plasma through Nondenaturing Gel Electrophoresis.

Comparative medicine

Napon G,Dafferner AJ,Saxena A,Lockridge O

MA3-042 was used in Immunohistochemistry to identify the major esterases in guinea pig plasma.

Mon Oct 01 00:00:00 UTC 2018

Neuromuscular synapse degeneration without muscle function loss in the diaphragm of a murine model for Huntington's Disease.

Neurochemistry international

Valadão PAC,Gomes MPSM,Aragão BC,Rodrigues HA,Andrade JN,Garcias R,Joviano-Santos JV,Luiz MA,Camargo WL,Naves LA,Kushmerick C,Cavalcante WLG,Gallacci M,de Jesus ICG,Guatimosim S,Guatimosim C

MA3-042 was used in Western Blot to contribute to expanding the current understanding of the effects of mutated huntingtin in the neuromuscular synapse and the diaphragm muscle function.

Fri Jun 01 00:00:00 UTC 2018

Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure.

Chemical research in toxicology

Dafferner AJ,Schopfer LM,Xiao G,Cashman JR,Yerramalla U,Johnson RC,Blake TA,Lockridge O

MA3-042 was used in Western Blotting to develop a method of immunopurifying human red blood cell acetylcholinesterase in quantities adequate for detecting exposure to nerve agents and organophosphorus pesticides, by mass spectrometry.

Mon Oct 16 00:00:00 UTC 2017

Functional variability in butyrylcholinesterase activity regulates intrathecal cytokine and astroglial biomarker profiles in patients with Alzheimer's disease.

Neurobiology of aging

Darreh-Shori T,Vijayaraghavan S,Aeinehband S,Piehl F,Lindblom RP,Nilsson B,Ekdahl KN,Långström B,Almkvist O,Nordberg A

MA3-042 was used in ELISA to study the effect of Alzheimer's disease patient butyrylcholinesterase genotype on the profile of inflammatory cytokines and markers of astroglial function in CSF

Fri Nov 01 00:00:00 UTC 2013

Lesions of cortical GABAergic interneurons and acetylcholine neurons in xeroderma pigmentosum group A.

Brain & development

Hayashi M,Ohto T,Shioda K,Fukatsu R

MA3-042 was used in immunohistochemistry to study the status of cortical GABAergic interneurons and cholinergic neurons in xeroderma pigmentosum group A

Sun Apr 01 00:00:00 UTC 2012

Decrease in acetylcholinergic neurons in the pedunculopontine tegmental nucleus in a patient with Prader-Willi syndrome.

Neuropathology : official journal of the Japanese Society of Neuropathology

Hayashi M,Miyata R,Tanuma N

MA3-042 was used in immunohistochemistry to study the selective deficiency of acetylcholine neurons in the pedunculopontine tegmental nucleus with Prader-Willi syndrome

Wed Jun 01 00:00:00 UTC 2011

Altered levels of acetylcholinesterase in Alzheimer plasma.

PloS one

García-Ayllón MS,Riba-Llena I,Serra-Basante C,Alom J,Boopathy R,Sáez-Valero J

MA3-042 was used in immunoprecipitation to investigate the changes of acetylcholinesterase level in Alzheimer plasma

Thu Jan 14 00:00:00 UTC 2010

Altered levels of acetylcholinesterase in Alzheimer plasma.

PloS one

García-Ayllón MS,Riba-Llena I,Serra-Basante C,Alom J,Boopathy R,Sáez-Valero J

MA3-042 was used in immunoprecipitation to investigate the changes of acetylcholinesterase level in Alzheimer plasma

Thu Jan 14 00:00:00 UTC 2010

Different cholinesterase inhibitor effects on CSF cholinesterases in Alzheimer patients.

Current Alzheimer research

Nordberg A,Darreh-Shori T,Peskind E,Soininen H,Mousavi M,Eagle G,Lane R

MA3-042 was used in western blot to investigate the effect of cholinesterase inhibitors on the actvities and protein levels of cholinesterases

Sun Feb 01 00:00:00 UTC 2009

Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation.

Molecular and cellular biology

Silveyra MX,Evin G,Montenegro MF,Vidal CJ,Martínez S,Culvenor JG,Sáez-Valero J

Published figure using Acetylcholinesterase monoclonal antibody (Product # MA3-042) in Western Blot

Thu May 01 00:00:00 UTC 2008

Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation.

Molecular and cellular biology

Silveyra MX,Evin G,Montenegro MF,Vidal CJ,Martínez S,Culvenor JG,Sáez-Valero J

Published figure using Acetylcholinesterase monoclonal antibody (Product # MA3-042) in Western Blot

Thu May 01 00:00:00 UTC 2008

Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation.

Molecular and cellular biology

Silveyra MX,Evin G,Montenegro MF,Vidal CJ,Martínez S,Culvenor JG,Sáez-Valero J

Published figure using Acetylcholinesterase monoclonal antibody (Product # MA3-042) in Western Blot

Thu May 01 00:00:00 UTC 2008

Fetal fornix transection and gestation length in sheep.

Experimental neurology

McDonald TJ,Li C,Vincent SE,Nijland MJ

MA3-042 was used in immunohistochemistry to study the developmental state of fetal hippocampus and its role in gestation

Tue Aug 01 00:00:00 UTC 2006

Immunocytochemical demonstration of axonal and perikaryal acetylcholinesterase in human cerebral cortex.

Brain research

Mesulam MM,Geula C,Cosgrove R,Mash D,Brimijoin S

MA3-042 was used in immunohistochemistry to investigate the expression of acetylcholinesterase in axons and perikarya in the human cerebral cortex

Fri Jan 25 00:00:00 UTC 1991

Bioinformatics

Protein Aliases: Acetylcholinesterase; acetylcholinesterase (Yt blood group); acetylcholinesterase T-subunit; AChE; apoptosis-related acetylcholinesterase; EC 3.1.1.7; OTTHUMP00000211349; OTTHUMP00000211356; Yt blood group

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Gene Aliases: ACEE; ACHE; ARACHE; N-ACHE; YT

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UniProt ID: (Human) P22303, (Bovine) P23795, (Cat) O62763, (Mouse) P21836

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Entrez Gene ID: (Rabbit) 100009390, (Human) 43, (Bovine) 540446, (Cat) 493674, (Mouse) 11423, (Guinea pig) 100422792

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Function(s)

beta-amyloid binding acetylcholinesterase activity cholinesterase activity protein binding collagen binding hydrolase activity serine hydrolase activity acetylcholine binding protein homodimerization activity laminin binding protein self-association choline binding carboxylic ester hydrolase activity esterase

Process(es)

acetylcholine catabolic process in synaptic cleft regulation of receptor recycling osteoblast development DNA replication acetylcholine catabolic process phosphatidylcholine biosynthetic process cell adhesion nervous system development synapse assembly muscle organ development cell proliferation response to wounding receptor internalization negative regulation of synaptic transmission, cholinergic neurotransmitter biosynthetic process amyloid precursor protein metabolic process neurotransmitter receptor biosynthetic process positive regulation of protein secretion protein tetramerization retina development in camera-type eye neurotransmitter catabolic process acetylcholine metabolic process choline metabolic process positive regulation of axonogenesis positive regulation of dendrite morphogenesis neuron death

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Acetylcholinesterase Monoclonal Antibody (HR2)

Acetylcholinesterase Antibody (MA3-042) in ICC/IF

Product Details

MA3-042

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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