|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000-1:15,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 6 publications below|
This antibody will not work in ICC/IF applications.
Suggested positive control: Hela whole cell extract.
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are hightly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Dose-dependent autophagic effect of titanium dioxide nanoparticles in human HaCaT cells at non-cytotoxic levels.
PA1-16889 was used in western blot to characterize the effect of dose-dependent autophagy and titanium dioxide nanoparticles in human HaCaT cells at non-cytotoxic levels
|Lopes VR,Loitto V,Audinot JN,Bayat N,Gutleb AC,Cristobal S||Journal of nanobiotechnology (14:null)||2016|
Identification of the epigenetic reader CBX2 as a potential drug target in advanced prostate cancer.
PA1-16889 was used in western blot to determine a potential drug target in advanced prostate cancer by identifying the epigenetic reader CBX2
|Clermont PL,Crea F,Chiang YT,Lin D,Zhang A,Wang JZ,Parolia A,Wu R,Xue H,Wang Y,Ding J,Thu KL,Lam WL,Shah SP,Collins CC,Wang Y,Helgason CD||Clinical epigenetics (8:null)||2016|
MYB controls erythroid versus megakaryocyte lineage fate decision through the miR-486-3p-mediated downregulation of MAF.
PA1-16889 was used in western blot to demonstrate that MYB affects lineage fate decision through the transactivation of miR-486-3p expression and MAF downregulation.
|Bianchi E,Bulgarelli J,Ruberti S,Rontauroli S,Sacchi G,Norfo R,Pennucci V,Zini R,Salati S,Prudente Z,Ferrari S,Manfredini R||Cell death and differentiation (22:1906)||2015|
Evaluation of PAX5 gene in the early stages of leukemic B cells in the childhood B cell acute lymphoblastic leukemia.
PA1-16889 was used in western blot to study the expression of the PAX5 gene and the presence of transactivation domain mutations in B cells of childhood B cell acute lymphoblastic leukemia
|Firtina S,Sayitoglu M,Hatirnaz O,Erbilgin Y,Oztunc C,Cinar S,Yildiz I,Celkan T,Anak S,Unuvar A,Devecioglu O,Timur C,Aydogan G,Akcay A,Atay D,Turkkan E,Karaman S,Orhaner B,Sarper N,Deniz G,Ozbek U||Leukemia research (36:87)||2012|
DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes.
PA1-16889 was used in western blot to study the role of DGAT enzymes in triacylglycerol synthesis and lipid droplets in adipocytes
|Harris CA,Haas JT,Streeper RS,Stone SJ,Kumari M,Yang K,Han X,Brownell N,Gross RW,Zechner R,Farese RV||Journal of lipid research (52:657)||2011|
The lysine demethylase LSD1 (KDM1) is required for maintenance of global DNA methylation.
PA1-16889 was used in western blot to investigate the role of KDM1 in the regulation of DNA methylation
|Wang J,Hevi S,Kurash JK,Lei H,Gay F,Bajko J,Su H,Sun W,Chang H,Xu G,Gaudet F,Li E,Chen T||Nature genetics (41:125)||2009|