This antibody reacts with all six known vertebrate isoforms of actin (MW ~42 kDa), as well as cytoplasmic actin beta and actin gamma. This antibody is highly recommended for monitoring total protein load on Western blots.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: a-actin; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 1, skeletal muscle; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; actin, gamma 2, smooth muscle, enteric; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; alpha skeletal muscle; alpha skeletal muscle actin; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; beta cytoskeletal actin; Beta-actin; cardiac actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; Gamma-2-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; PS1TP5-binding protein 1; skeletal alpha actin; smooth muscle alpha-actin; Smooth muscle gamma-actin; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; ACT-5; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG2; ACTGE; actin; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; alphaSMA; ASD5; ASMA; Bact; beta-actin; BOS_10523; BOS_11389; BOS_22932; BOS_23286; BOS_23966; BRWS1; CFTD; CFTD1; CFTDM; CMD1R; CMH11; E430023M04Rik; GAMMA-ACTIN; GIG46; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; RCJMB04_4h19; SHPM; SMalphaA; SMGA; VSCM
UniProt ID: (Chicken) P68139, (Human) P68133, (Bovine) P68138, (Pig) P68137, (Rabbit) P62740, (Chicken) P08023, (Bovine) P62739, (Human) P62736, (Rabbit) P29751, (Bovine) P60712, (Pig) Q6QAQ1, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Human) P68032, (Bovine) Q3ZC07, (Chicken) P68034, (Bovine) Q5E9B5, (Chicken) P63270, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) Q64316, (Rat) P68035, (Mouse) P68033, (Mouse) P63268, (Rat) P63269
Entrez Gene ID: (Chicken) 421534, (Human) 58, (Bovine) 281592, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Rabbit) 100009272, (Bovine) 280979, (Pig) 414396, (Human) 60, (Chicken) 396526, (Dog) 403580, (Dog) 478250, (Human) 70, (Bovine) 533219, (Chicken) 423298, (Rabbit) 100340339, (Pig) 100152267, (Bovine) 281595, (Chicken) 396084, (Human) 72, (Rabbit) 100350318, (Dog) 475792, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11468, (Rat) 25365, (Rabbit) 100009506