MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.
MA5-11869 is a suggested replacement to Product # 82353, clone # C4.
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: a-actin; actin; actin alpha 1; Actin, alpha skeletal muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; alpha actin 1; alpha actin 2; alpha skeletal muscle; alpha skeletal muscle actin; Alpha-actin; Alpha-actin-1; Alpha-actin-2; alpha-cardiac actin; alpha-smooth muscle actin; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; nemaline myopathy type 3; skeletal alpha actin; smooth muscle alpha-actin; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT-4; ACTA; Acta-2; ACTA1; ACTA2; Acts; ACTSA; Actsk-1; ACTVS; alphaSMA; ASMA; BOS_23286; BOS_23966; CFTD; CFTD1; CFTDM; GIG46; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA
UniProt ID: (Chicken) P68139, (Human) P68133, (Bovine) P68138, (Pig) P68137, (Rabbit) P62740, (Chicken) P08023, (Bovine) P62739, (Human) P62736, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737
Entrez Gene ID: (Chicken) 421534, (Human) 58, (Bovine) 281592, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Dog) 477587, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rabbit) 100009506