|Tested species reactivity||Bovine, Dog, Chicken, Fruit fly, Cat, Guinea pig, Human, Leech, Mouse, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Dog, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to N-terminus of human beta-actin|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16914 targets Actin Beta in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Chicken, Drosophila, Feline, Guinea Pig, Human, Leech, mouse, Ovine, Porcine, Rabbit, and Rat samples.
The PA5-16914 immunogen is synthetic peptide corresponding to N-terminus of human beta-actin.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three alpha-actin (skeletal, cardiac, and smooth muscle), one beta-actin (beta nonmuscle), and two gamma-actins (gamma-smooth nuscle and gamma-nonmuscle). Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Therapeutic Potential of Dental Pulp Stem Cell Secretome for Alzheimer's Disease Treatment: An In Vitro Study.
PA5-16914 was used in western blot to evaluate the therapeutic use of the dental pulp stem cell secretome to reduce cytotoxicity and apoptosis caused by the amyloid beta peptide
|Ahmed Nel-M,Murakami M,Hirose Y,Nakashima M||Stem cells international (2016:null)||2016|
Influence of Matrices on 3D-Cultured Prostate Cancer Cells' Drug Response and Expression of Drug-Action Associated Proteins.
PA5-16914 was used in western blot to characterize 3D-cultured prostate cancer cells' drug response and expression of drug-action associated proteins and the influence of matrices
|Edmondson R,Adcock AF,Yang L||PloS one (11:null)||2016|
|Not Applicable||Not Cited||
Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1.
PA5-16914 was used in western blot to characterize a feedforward mechanism at the G protein-coupled estrogen receptor 1 that controls estrogen enhaced linkage in the vascular endothelial calmodulin network
|Tran QK,Firkins R,Giles J,Francis S,Matnishian V,Tran P,VerMeer M,Jasurda J,Burgard MA,Gebert-Oberle B||The Journal of biological chemistry (291:10805)||2016|
Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue.
PA5-16914 was used in western blot determine the effects of cryopreservation on canine adipose-derived multipotent stromal cells
|Duan W,Lopez MJ||Stem cell reviews (12:257)||2016|
Age-dependent decline in dental pulp regeneration after pulpectomy in dogs.
PA5-16914 was used in western blot to study the cellular mechanisms underlying the age-dependent decrease in the regenerative capacity of canine dental pulp
|Iohara K,Murakami M,Nakata K,Nakashima M||Experimental gerontology (52:39)||2014|
The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.
PA5-16914 was used in western blot to develop a method for the isolation of high regenerative potential dental pulp stem cells based on G-CSF-induced migration
|Murakami M,Horibe H,Iohara K,Hayashi Y,Osako Y,Takei Y,Nakata K,Motoyama N,Kurita K,Nakashima M||Biomaterials (34:9036)||2013|
NLRP3 inflammasome blockade inhibits VEGF-A-induced age-related macular degeneration.
PA5-16914 was used in western blot to study age-related macular degeneration and the roles played by VEGF-A and the NLRP3 inflammasome
|Marneros AG||Cell reports (4:945)||2013|
Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.
PA5-16914 was used in western blot to study the anti-tumor effects of a system for the GR-mediated delivery of anti-Hsp90 miRNA
|Pore SK,Choudhary A,Rathore B,Ganguly A,Sujitha P,Kumar CG,Agawane SB,Kumar JM,Scaria V,Pillai B,Banerjee R||Biomaterials (34:6804)||2013|
|Not Applicable||Not Cited||
Immunolocalization of skeletal matrix proteins in tissue and mineral of the coral Stylophora pistillata.
PA5-16914 was used in immunohistochemistry - paraffin section to show the spatial arrangement of key matrix proteins in tissue and skeleton of Stylophora pistillata
|Mass T,Drake JL,Peters EC,Jiang W,Falkowski PG||Proceedings of the National Academy of Sciences of the United States of America (111:12728)||2014|