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Invitrogen
Actin Muscle Monoclonal Antibody (HHF35)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-10046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Cat,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Published species
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
HHF35
Immunogen
SDS extracted protein fraction of human myocardium
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.
Western Blot: Reducing conditions; Immunohistochemistry (Paraffin): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a-actin; ACTA1; ACTA2; ACTC; ACTG2; actin; actin alpha 1; actin alpha cardiac; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; actin, cytoplasmic 2; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin-like protein; alpha-actin; alpha-actin cardiac; Alpha-actin-1; alpha-actin-2; alpha-actin-3; alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; beta-actin FE-3; cardiac actin; cell growth-inhibiting gene 46 protein; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal gamma-actin; epididymis luminal protein 176; gamma 1 propeptide; gamma-2-actin; gamma-actin; gamma-enteric smooth muscle actin; GIG46; nemaline myopathy type 3; RP5-1068B5.2; skeletal muscle alpha-actin; smooth muscle alpha-actin; smooth muscle gamma-actin; type 5; unnamed protein product
Gene Aliases: 0610041G09Rik; a-SMA; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA3; ACTC; Actc-1; ACTE; ACTG; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; alphaSMA; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; DFNA20; DFNA26; E51; GAMMA-ACTIN; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; SHPM; SMAalpha; SMalphaA; SMDYS; SMGA; VSCM; VSCM1
UniProt ID: (Human) P68133, (Chicken) P68139, (Rabbit) P68135, (Rabbit) P62740, (Chicken) P08023, (Human) P62736, (Chicken) P68034, (Human) P68032, (Chicken) P53478, (Human) P63261, (Chicken) P63270, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Rat) P63269, (Mouse) P63268
Entrez Gene ID: (Human) 58, (Chicken) 421534, (Rabbit) 100009506, (Dog) 488984, (Dog) 477587, (Rabbit) 100009271, (Chicken) 423787, (Human) 59, (Cat) 100144392, (Dog) 478250, (Chicken) 423298, (Human) 70, (Rabbit) 100340339, (Chicken) 415296, (Dog) 475923, (Human) 71, (Chicken) 396084, (Human) 72, (Rabbit) 100350318, (Dog) 475792, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Rat) 25365, (Mouse) 11468
structural constituent of cytoskeleton
protein binding
ATP binding
ATPase activity
myosin binding
ADP binding
protein kinase binding
microfilament motor activity
structural constituent of postsynaptic actin cytoskeleton
profilin binding
ubiquitin protein ligase binding
identical protein binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
muscle contraction
positive regulation of gene expression
skeletal muscle thin filament assembly
skeletal muscle fiber development
mesenchyme migration
cytoskeleton organization
response to virus
positive regulation of hepatic stellate cell migration
positive regulation of hepatic stellate cell contraction
positive regulation of ERK1 and ERK2 cascade
cellular response to transforming growth factor beta stimulus
juxtaglomerular apparatus development
glomerular mesangial cell development
positive regulation of hepatic stellate cell activation
actin filament organization
actin-myosin filament sliding
heart contraction
actin filament-based movement
actomyosin structure organization
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
axonogenesis
cell motility
postsynaptic actin cytoskeleton organization
angiogenesis
morphogenesis of a polarized epithelium
positive regulation of cell migration
maintenance of blood-brain barrier
regulation of stress fiber assembly
regulation of focal adhesion assembly
platelet aggregation
positive regulation of wound healing
tight junction assembly
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
protein localization to bicellular tight junction
response to ethanol
cellular response to interleukin-6
cellular response to acetaldehyde
response to mechanical stimulus
cellular response to potassium ion
skeletal muscle fiber adaptation
response to steroid hormone
regulation of blood pressure
vascular smooth muscle contraction
response to xenobiotic stimulus
negative regulation of apoptotic process
cardiac muscle contraction
cytoplasmic actin-based contraction involved in cell motility
sarcomere organization
cellular response to interferon-gamma
response to calcium ion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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