Invitrogen

Actin Muscle Monoclonal Antibody (HHF35)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

11 References

View all (6) Actin Muscle antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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Actin Muscle Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry staining of human smooth muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35) Monoclonal antibody (Product # MA1-10046).

Actin Muscle Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Rat Colon (Lane 6), Rat Pancreas (Lane 7), Mouse Kidney (Lane 8) and whole cell extract (30 µg lysate) of C2C12 (Lane 5). The blot was probed with Anti-Actin Muscle Monoclonal Antibody (HHF35) (Product # MA1-10046, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to beta Actin was observed in muscle specific tissues and cell line when compared to non muscle tissues.

Actin Muscle Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of Actin Muscle was observed in Mouse Heart, Rat Heart, Mouse Skeletal Muscle, Rat Skeletal Muscle and C2C12 in comparison to Rat Colon, Rat Pancreas and Mouse Kidney using product (Product # MA1-10046) in western blot. {RE}

Product Details

MA1-10046

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 9 publications 9 publications

Product Specifications
Species Reactivity	Dog, Chicken, Cat, Human, Mouse, Non-human primate, Rabbit, Rat
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	HHF35
Immunogen	SDS extracted protein fraction of human myocardium
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	15mM sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_11154239

Product Specific Information

This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Western Blot: Reducing conditions; Immunohistochemistry (Paraffin): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.

International journal of surgical pathology

Lee PW,Yau DT,Lau PP,Chan JK

MA1-10046 was used in immunohistochemistry to report on a case of plexiform fibromyxoma of the stomach

Thu May 01 00:00:00 EDT 2014

Expression of E-cadherin in angiomyolipoma.

Human pathology

Wang Z,Gong Q,Fan Q

MA1-10046 was used in immunohistochemistry to perform a systematic study of E-cadherin expression in angiomyolipoma

Sat Dec 01 00:00:00 EST 2012

Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.

Diagnostic pathology

Yin WH,Li J,Chan JK

MA1-10046 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression

Thu Apr 12 00:00:00 EDT 2012

Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?

International journal of surgical pathology

Eloy C,Vinagre J,Cameselle-Teijeiro J,Paiva ME,Soares P,Sobrinho-Simões M

MA1-10046 was used in immunohistochemistry to report on a case of tumor-in-tumor of the thyroid with basaloid differentiation

Fri Apr 01 00:00:00 EDT 2011

G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.

Cell stem cell

Shimoji K,Yuasa S,Onizuka T,Hattori F,Tanaka T,Hara M,Ohno Y,Chen H,Egasgira T,Seki T,Yae K,Koshimizu U,Ogawa S,Fukuda K

MA1-10046 was used in immunocytochemistry to investigate the expression level of G-CSFR in vivo and the role of G-CSF during cardiomyogenesis in vitro and in vivo

Fri Mar 05 00:00:00 EST 2010

Chorangiosis: the potential role of smoking and air pollution.

Pathology, research and practice

Akbulut M,Sorkun HC,Bir F,Eralp A,Duzcan E

MA1-10046 was used in immunohistochemistry to investigate the role of smoking and air pollution in the development of chorangiosis

Tue May 19 00:00:00 EDT 2009

Microfabrication-based modulation of embryonic stem cell differentiation.

Lab on a chip

Park J,Cho CH,Parashurama N,Li Y,Berthiaume F,Toner M,Tilles AW,Yarmush ML

MA1-10046 was used in immunocytochemistry to study the effect of microfabricated adhesive stencils on embryonic stem cell differentiation

Wed Aug 01 00:00:00 EDT 2007

Myointimoma of the glans penis.

Pathology international

Vardar E,Gunlusoy B,Arslan M,Kececi S

MA1-10046 was used in immunohistochemistry to report on a case of myointimoma of the glans penis

Thu Mar 01 00:00:00 EST 2007

Ectopic cervical anaplastic ependymoma.

Pathology international

Wang Z,Huang G,Yan P,Liang R,Wang J,Yan Q,Zhang J,Cheng H,Hu P,Ma MJ

MA1-10046 was used in immunohistochemistry to report on a case of ectopic cervical anaplastic ependymoma

Thu Dec 01 00:00:00 EST 2005

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA1-10046 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 EDT 2005

Epstein-Barr virus-associated smooth muscle tumour: a distinctive mesenchymal tumour of immunocompromised individuals.

Pathology

Cheuk W,Li PC,Chan JK

MA1-10046 was used in immunohistochemistry to report on a case of Epstein-Barr virus-associated smooth muscle tumour

Sat Jun 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: a-actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; beta-actin FE-3; cardiac actin; Cell growth-inhibiting gene 46 protein; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; gamma 1 propeptide; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin; type 5; unnamed protein product

Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GAMMA-ACTIN; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMGA; VSCM

UniProt ID: (Chicken) P68139, (Human) P68133, (Rabbit) P62740, (Chicken) P08023, (Human) P62736, (Human) P68032, (Chicken) P68034, (Human) P63261, (Chicken) P53478, (Chicken) P63270, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P99022, (Mouse) P63268, (Rat) P63269