Invitrogen

Actin Muscle Monoclonal Antibody (HHF35)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

13 References

View all (6) Actin Muscle antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

Actin Muscle Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Actin Muscle Specific (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin Muscle Specific monoclonal antibody (Product # MA5-14084) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

Actin Muscle Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human skeletal Muscle stained with Muscle Specific Actin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of muscle cells.

Actin Muscle Antibody in Western Blot (WB) — Western blot analysis of Actin Muscle Specific was performed by loading 25 µg of mouse skeletal muscle (Lane 1), Raji (Lane 2) and Hela (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Actin Muscle Specific monoclonal antibody (Product # MA5-14084) at a dilution of 1:500 (mouse) and 1:100 (Raji and Hela) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 42 kDa.

Actin Muscle Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of Actin Muscle was observed in muscle tissues (skeletal muscle and heart) in comparison to non muscle tissues (brain and liver) using (Product # MA5-14084) in western blot. {RE}

Product Details

MA5-14084

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100-1:500	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 10 publications 10 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	HHF35
Immunogen	SDS extract of human myocardium
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	tissue culture supernatant
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10975398

Product Specific Information

This antibody reacts with actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart and smooth muscle cells. It recognizes alpha-actin from skeletal, cardiac, and smooth muscle, and reacts with gamma actin from smooth muscle. This antibody will react with tumors from smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle tumors (rhabdomyomas and rhabdomyosarcomas).

Staining of formalin-fixed tissues requires boiling in 1mM EDTA, pH 8.0, followed by cooling at room temperature for 20 min.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evidence for CD34/SMA positive cells in the left main coronary artery in atherogenesis.

Acta histochemica

Kruzliak P,Hare DL,Sabaka P,Delev D,Gaspar L,Rodrigo L,Zulli A

MA5-14084 was used in immunohistochemistry - paraffin section to study the evidence of CD34/SMA positive cells in atherogenesis in left main coronary artery

Sun May 01 00:00:00 EDT 2016

Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.

International journal of surgical pathology

Lee PW,Yau DT,Lau PP,Chan JK

MA5-14084 was used in immunohistochemistry to report on a case of plexiform fibromyxoma of the stomach

Thu May 01 00:00:00 EDT 2014

Expression of E-cadherin in angiomyolipoma.

Human pathology

Wang Z,Gong Q,Fan Q

MA5-14084 was used in immunohistochemistry to perform a systematic study of E-cadherin expression in angiomyolipoma

Sat Dec 01 00:00:00 EST 2012

Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.

Diagnostic pathology

Yin WH,Li J,Chan JK

MA5-14084 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression

Thu Apr 12 00:00:00 EDT 2012

Fascin expression and its potential significance in gastrointestinal stromal tumors.

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology

Ozcan A,Karslioğlu Y,Günal A,Cermık AH,Kurt B,Ongürü O

MA5-14084 was used in immunohistochemistry to study the expression of fascin in gastrointestinal stromal tumors and its possible prognostic value

Mon Aug 01 00:00:00 EDT 2011

Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?

International journal of surgical pathology

Eloy C,Vinagre J,Cameselle-Teijeiro J,Paiva ME,Soares P,Sobrinho-Simões M

MA5-14084 was used in immunohistochemistry to report on a case of tumor-in-tumor of the thyroid with basaloid differentiation

Fri Apr 01 00:00:00 EDT 2011

G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.

Cell stem cell

Shimoji K,Yuasa S,Onizuka T,Hattori F,Tanaka T,Hara M,Ohno Y,Chen H,Egasgira T,Seki T,Yae K,Koshimizu U,Ogawa S,Fukuda K

MA5-14084 was used in immunocytochemistry to investigate the expression level of G-CSFR in vivo and the role of G-CSF during cardiomyogenesis in vitro and in vivo

Fri Mar 05 00:00:00 EST 2010

Chorangiosis: the potential role of smoking and air pollution.

Pathology, research and practice

Akbulut M,Sorkun HC,Bir F,Eralp A,Duzcan E

MA5-14084 was used in immunohistochemistry to investigate the role of smoking and air pollution in the development of chorangiosis

Tue May 19 00:00:00 EDT 2009

Microfabrication-based modulation of embryonic stem cell differentiation.

Lab on a chip

Park J,Cho CH,Parashurama N,Li Y,Berthiaume F,Toner M,Tilles AW,Yarmush ML

MA5-14084 was used in immunocytochemistry to study the effect of microfabricated adhesive stencils on embryonic stem cell differentiation

Wed Aug 01 00:00:00 EDT 2007

Myointimoma of the glans penis.

Pathology international

Vardar E,Gunlusoy B,Arslan M,Kececi S

MA5-14084 was used in immunohistochemistry to report on a case of myointimoma of the glans penis

Thu Mar 01 00:00:00 EST 2007

Ectopic cervical anaplastic ependymoma.

Pathology international

Wang Z,Huang G,Yan P,Liang R,Wang J,Yan Q,Zhang J,Cheng H,Hu P,Ma MJ

MA5-14084 was used in immunohistochemistry to report on a case of ectopic cervical anaplastic ependymoma

Thu Dec 01 00:00:00 EST 2005

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA5-14084 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 EDT 2005

Epstein-Barr virus-associated smooth muscle tumour: a distinctive mesenchymal tumour of immunocompromised individuals.

Pathology

Cheuk W,Li PC,Chan JK

MA5-14084 was used in immunohistochemistry to report on a case of Epstein-Barr virus-associated smooth muscle tumour

Sat Jun 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin

Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMGA; VSCM

UniProt ID: (Human) P68133, (Human) P62736, (Human) P68032, (Human) P63261, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Mouse) P68033, (Rat) P68035, (Mouse) P63260, (Rat) P99022, (Mouse) P63268, (Rat) P63269