Immunofluorescence analysis of Actin Skeletal Muscle was done on 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Actin Skeletal Muscle [5C5.F8.C7 (alpha-Sr-1)] Mouse Monoclonal Antibody (MA512542) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Amphibian, Bovine, Guinea pig, Human, Mouse, Sheep, Rabbit, Rat|
|Published species reactivity||Rabbit, Human|
|Host / Isotype||Mouse / IgM, kappa|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-12542 targets Actin Skeletal Muscle in IF and IHC (P) applications and shows reactivity with Amphibian, Bovine, Guinea Pig, Human, Ovine, Rabbit, and Rat samples.
The MA5-12542 immunogen is n-terminal decapeptide.
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Epithelial and muscular regionalization of the human developing anorectum.
MA5-12542 was used in immunohistochemistry to study epithelial and muscular regionalization during development of the human anorectum
|Fritsch H,Aigner F,Ludwikowski B,Reinstadler-Zankl S,Illig R,Urbas D,Schwarzer C,Longato S||Anatomical record (Hoboken, N.J. : 2007) (290:1449)||2007|
Caveolin-1 expression is associated with plaque formation in hypercholesterolemic rabbits.
MA5-12542 was used in immunohistochemistry to study the association of caveolin-1 expression with plaque formation in hypercholesterolemic rabbits
|Lin WW,Lin YC,Chang TY,Tsai SH,Ho HC,Chen YT,Yang VC||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (54:897)||2006|
Spindle cell lipoma in an intramuscular lipoma.
MA5-12542 was used in immunohistochemistry to study a spindle cell lipoma in an intramuscular lipoma
|Usta U,Türkmen E,Mizrak B,Yildiz D,Güzel Z||Pathology international (54:734)||2004|
Clear cell sarcoma of the stomach.
MA5-12542 was used in immunohistochemistry to report on a case of clear cell sarcoma of the stomach
|Pauwels P,Debiec-Rychter M,Sciot R,Vlasveld T,den Butter B,Hagemeijer A,Hogendoorn PC||Histopathology (41:526)||2002|