|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant fragment of human Activin Receptor Type IIA.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 5% trehalose|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||15 ug/ml|
|Neutralization (Neu)||1-3 ug/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20174 detects Activin Receptor Type IIA in human samples.
PA1-20174 has been successfully used in ELISA, neutralization assays, immunohistochemistry, and Western blot procedures. Neutralization: when used at a 1-3 ug/ml, the antibody will block 50% of the binding of 30 ng/ml recombinant human activin A to immobilized recombinant human activin receptor IIA/Fc chimera (100 ul of a 0.5 ug/ml solution coating each well) in a functional ELISA assay.
The PA1-20174 immunogen is a recombinant fragment of human Activin Receptor Type IIA.
Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. . To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splicevariants of activin receptor IIA and five splice variants of activin receptor IIB have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.