Invitrogen

Adenosine Deaminase Monoclonal Antibody (6D4)

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Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human rectal cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.

Adenosine Deaminase Antibody in Western Blot (WB) — Western blot analysis of Adenosine Deaminase in Lane 1: human HeLa whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) using a 0.5 µg/mL dilution, followed by a goat anti-mouse IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Adenosine Deaminase Antibody in Flow Cytometry (Flow) — Flow cytometry of Adenosine Deaminase in U20S cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-mouse IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6D4

Immunogen

E.coli-derived human ADA recombinant protein (Position: Q135-L363).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

0.2mg sodium phosphate with 4mg trehalose, 0.9mg NaCl

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Adenosine aminohydrolase; Adenosine deaminase

Gene Aliases: ADA; ADA1

UniProt ID: (Human) P00813

Entrez Gene ID: (Human) 100

Molecular Function: deaminase hydrolase

Function(s)

Process(es)

purine nucleoside binding adenosine deaminase activity protein binding zinc ion binding

Adenosine Deaminase Monoclonal Antibody (6D4)

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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