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Adenosine Deaminase Antibody (MA5-33006) in WB
Western blot analysis of Adenosine Deaminase in Lane 1: human HeLa whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature).... View More
Product Details
MA5-33006
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6D4
Immunogen
E.coli-derived human ADA recombinant protein (Position: Q135-L363).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2802640
Product Specific Information
Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenosine aminohydrolase; Adenosine deaminase; RP11-61L14.5
Gene Aliases: ADA; ADA1
UniProt ID: (Human) P00813
Entrez Gene ID: (Human) 100
Molecular Function:
deaminase
response to hypoxia
histamine secretion
trophectodermal cell differentiation
liver development
placenta development
germinal center B cell differentiation
positive regulation of germinal center formation
negative regulation of leukocyte migration
negative regulation of mature B cell apoptotic process
adenosine catabolic process
deoxyadenosine catabolic process
aging
purine ribonucleoside monophosphate biosynthetic process
positive regulation of heart rate
positive regulation of B cell proliferation
purine nucleotide salvage
positive regulation of T cell differentiation in thymus
response to vitamin E
regulation of cell-cell adhesion mediated by integrin
T cell activation
negative regulation of circadian sleep/wake cycle, non-REM sleep
response to drug
response to hydrogen peroxide
purine-containing compound salvage
hypoxanthine salvage
response to morphine
positive regulation of smooth muscle contraction
dATP catabolic process
inosine biosynthetic process
xanthine biosynthetic process
positive regulation of alpha-beta T cell differentiation
lung alveolus development
Peyer's patch development
embryonic digestive tract development
negative regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive regulation of T cell receptor signaling pathway
negative regulation of adenosine receptor signaling pathway
negative regulation of penile erection
negative regulation of thymocyte apoptotic process
negative regulation of mucus secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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