Invitrogen

Adenosine Deaminase Monoclonal Antibody (6D4)

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Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human rectal cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.

Adenosine Deaminase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human tonsil tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.

Adenosine Deaminase Antibody in Western Blot (WB) — Western blot analysis of Adenosine Deaminase in Lane 1: human HeLa whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) using a 0.5 µg/mL dilution, followed by a goat anti-mouse IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Adenosine Deaminase Antibody in Flow Cytometry (Flow) — Flow cytometry of Adenosine Deaminase in U20S cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-mouse IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.

Product Details

MA5-33006

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
Flow Cytometry (Flow)	1-3 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	6D4
Immunogen	E.coli-derived human ADA recombinant protein (Position: Q135-L363). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2802640

Product Specific Information

Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Adenosine aminohydrolase; Adenosine deaminase; RP11-61L14.5

Gene Aliases: ADA; ADA1

UniProt ID: (Human) P00813

Entrez Gene ID: (Human) 100

Molecular Function: deaminase

response to hypoxia histamine secretion trophectodermal cell differentiation liver development placenta development germinal center B cell differentiation positive regulation of germinal center formation negative regulation of leukocyte migration negative regulation of mature B cell apoptotic process adenosine catabolic process deoxyadenosine catabolic process aging purine ribonucleoside monophosphate biosynthetic process positive regulation of heart rate positive regulation of B cell proliferation purine nucleotide salvage positive regulation of T cell differentiation in thymus response to vitamin E regulation of cell-cell adhesion mediated by integrin T cell activation negative regulation of circadian sleep/wake cycle, non-REM sleep response to drug response to hydrogen peroxide purine-containing compound salvage hypoxanthine salvage response to morphine positive regulation of smooth muscle contraction dATP catabolic process inosine biosynthetic process xanthine biosynthetic process positive regulation of alpha-beta T cell differentiation lung alveolus development Peyer's patch development embryonic digestive tract development negative regulation of inflammatory response positive regulation of calcium-mediated signaling positive regulation of T cell receptor signaling pathway negative regulation of adenosine receptor signaling pathway negative regulation of penile erection negative regulation of thymocyte apoptotic process negative regulation of mucus secretion

Process(es)

purine nucleoside binding adenosine deaminase activity protein binding zinc ion binding