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Immunofluorescence analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-041A detects adenosine receptor A1 (A1AR) in rat tissues. This antibody does not detect other AR subtypes. Previous lots of this antibody have been used in human and bovine samples.
PA1-041A has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects an ~37 kDa protein in rat brain representing A1AR.
The PA1-041A immunogen is a synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.
The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neonatal caffeine treatment up-regulates adenosine receptors in brainstem and hypothalamic cardio-respiratory related nuclei of rat pups.
PA1-041A was used in immunohistochemistry to study the effect of neonatal caffeine treatment on the brainstem and hypothalamic cardio-respiratory related nuclei of rat pups
|Gaytan SP,Pasaro R||Experimental neurology (237:247)||2012|
Attenuation of cerebral vasospasm and secondary injury by 17beta-estradiol following experimental subarachnoid hemorrhage.
PA1-041A was used in western blot to study the effect of 17beta-estradiol on cerebral vasospasm and secondary injury after subarachnoid hemorrhage
|Lin CL,Dumont AS,Su YF,Tsai YJ,Huang JH,Chang KP,Howng SL,Kwan AL,Kassell NF,Kao CH||Journal of neurosurgery (110:457)||2009|
Cell cycle-dependent regulation of SFK, JAK1 and STAT3 signalling by the protein tyrosine phosphatase TCPTP.
PA1-041A was used in western blot to study the effect of protein tyrosine phosphatase on SFK, JAK1 and STAT3 signalling
|Shields BJ,Court NW,Hauser C,Bukczynska PE,Tiganis T||Cell cycle (Georgetown, Tex.) (7:3405)||2008|
Adenosine A 2B receptors modulate cAMP levels and induce CREB but not ERK1/2 and p38 phosphorylation in rat skeletal muscle cells.
PA1-041A was used in western blot to investigate the role of adenosine A(2B) on specific signal pathway in rat skeletal muscle cells
|Lynge J,Schulte G,Nordsborg N,Fredholm BB,Hellsten Y||Biochemical and biophysical research communications (307:180)||2003|
A1AR; adenosine A1 receptor variant 1; adenosine A1 receptor variant 2; adenosine receptor A1