|Tested species reactivity||Virus|
|Published species reactivity||Virus, Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Clone||M58 + M73|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11349 targets Adenovirus Type 2 and 5 in IF, IHC (P), IP, and WB applications and shows reactivity with Virus samples.
The MA5-11349 immunogen is adenovirus.
Adenovirus Ab-5 detects adenovirus serogroups 2 and 5. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MicroRNA-mediated suppression of oncolytic adenovirus replication in human liver.
MA5-11349 was used in immunohistochemistry to study the incorporation of liver-specific miRNA-122 sequences into oncolytic adenoviruses as a means of inhibiting viral replication in normal liver whilst maintaining replication in cancer cells
|Ylösmäki E,Lavilla-Alonso S,Jäämaa S,Vähä-Koskela M,af Hällström T,Hemminki A,Arola J,Mäkisalo H,Saksela K||PloS one (8:null)||2013|
Biodistribution of an oncolytic adenovirus after intracranial injection in permissive animals: a comparative study of Syrian hamsters and cotton rats.
MA5-11349 was used in immunohistochemistry to study the biodistribution of an oncolytic adenovirus after intracranial injection in Syrian hamsters and cotton rats
|Sonabend AM,Ulasov IV,Han Y,Rolle CE,Nandi S,Cao D,Tyler MA,Lesniak MS||Cancer gene therapy (16:362)||2009|
Coxsackievirus B3 sequences in the myocardium of fatal cases in a cluster of acute myocarditis in Greece.
MA5-11349 was used in immunohistochemistry to study Coxsackievirus B3 sequences in the myocardium of fatal cases of acute myocarditis in Greece
|Spanakis N,Manolis EN,Tsakris A,Tsiodras S,Panagiotopoulos T,Saroglou G,Legakis NJ||Journal of clinical pathology (58:357)||2005|
Targeted cancer gene therapy using a hypoxia inducible factor dependent oncolytic adenovirus armed with interleukin-4.
MA5-11349 was used in western blot to study targeted cancer gene therapy using a HIF-1-dependent oncolytic adenovirus expressing interleukin-4
|Post DE,Sandberg EM,Kyle MM,Devi NS,Brat DJ,Xu Z,Tighiouart M,Van Meir EG||Cancer research (67:6872)||2007|