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|Tested species reactivity||Virus|
|Published species reactivity||Virus, Hamster, Human, Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||UV-irradiated Ad2 virus|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11222 targets Adenovirus Fiber in IF and WB applications and shows reactivity with Virus samples.
The MA5-11222 immunogen is uV-irradiated Ad2 virus.
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An adenovirus vector incorporating carbohydrate binding domains utilizes glycans for gene transfer.
MA5-11222 was used in western blot to study the use of carbohydrate binding domains to target human adenovirus serotype 5 vector-based gene transfer
|Kim JW,Glasgow JN,Nakayama M,Ak F,Ugai H,Curiel DT||PloS one (8:null)||2013|
Disulfide-bond formation by a single cysteine mutation in adenovirus protein VI impairs capsid release and membrane lysis.
MA5-11222 was used in western blot to study the role of a cysteine mutation in adenovirus protein VI in impairing capsid release and membrane lysis
|Moyer CL,Nemerow GR||Virology (428:41)||2012|
Derivation of a myeloid cell-binding adenovirus for gene therapy of inflammation.
MA5-11222 was used in western blot to develop a myeloid cell-binding adenovirus for gene therapy of inflammation
|Alberti MO,Roth JC,Ismail M,Tsuruta Y,Abraham E,Pereboeva L,Gerson SL,Curiel DT||PloS one (7:null)||2012|
Functional genetic and biophysical analyses of membrane disruption by human adenovirus.
MA5-11222 was used in western blot to study the genetics and biophysics of membrane disruption by human adenovirus
|Moyer CL,Wiethoff CM,Maier O,Smith JG,Nemerow GR||Journal of virology (85:2631)||2011|
An adenoviral vector expressing human adenovirus 5 and 3 fiber proteins for targeting heterogeneous cell populations.
MA5-11222 was used in western blot to generate an adenovirus expressing adenovirus 5 and 3 fibre proteins for targeting heterogeneous cell populations
|Murakami M,Ugai H,Wang M,Belousova N,Dent P,Fisher PB,Glasgow JN,Everts M,Curiel DT||Virology (407:196)||2010|
Chimeric adenoviral vectors incorporating a fiber of human adenovirus 3 efficiently mediate gene transfer into prostate cancer cells.
MA5-11222 was used in western blot to study the use of chimeric adenoviral vectors to mediate gene transfer into prostate cancer cells
|Murakami M,Ugai H,Belousova N,Pereboev A,Dent P,Fisher PB,Everts M,Curiel DT||The Prostate (70:362)||2010|
A strategy for adenovirus vector targeting with a secreted single chain antibody.
MA5-11222 was used in western blot to develop targeted adenovirus vectors utilizing a secreted single-chain antibody sequence
|Glasgow JN,Mikheeva G,Krasnykh V,Curiel DT||PloS one (4:null)||2009|
Insufficient accumulation of viral late mRNAs restricts the replicative cycle of human adenovirus type 37 in A549 cells.
MA5-11222 was used in western blot to study the replicative cycle of the poorly growing human adenovirus type 37 serotype
|Adachi K,Mitani K||Archives of virology (154:1401)||2009|
Treatment of pancreatic cancer with an oncolytic adenovirus expressing interleukin-12 in Syrian hamsters.
MA5-11222 was used in western blot to evaluate the therapeutic benefit of an oncolytic adenoviral interleukin-12 construct for pancreatic cancer treatment in an animal model
|Bortolanza S,Bunuales M,Otano I,Gonzalez-Aseguinolaza G,Ortiz-de-Solorzano C,Perez D,Prieto J,Hernandez-Alcoceba R||Molecular therapy : the journal of the American Society of Gene Therapy (17:614)||2009|
Improved retinal transduction in vivo and photoreceptor-specific transgene expression using adenovirus vectors with modified penton base.
MA5-11222 was used in western blot to develop adenoviral vectors with improved retinal transduction in vivo and photoreceptor-specific transgene expression
|Cashman SM,McCullough L,Kumar-Singh R||Molecular therapy : the journal of the American Society of Gene Therapy (15:1640)||2007|
Adenovirus fiber shaft contains a trimerization element that supports peptide fusion for targeted gene delivery.
MA5-11222 was used in western blot to study a novel trimerization element in the adenovirus fiber shaft with potential for targeted gene delivery
|Li J,Lad S,Yang G,Luo Y,Iacobelli-Martinez M,Primus FJ,Reisfeld RA,Li E||Journal of virology (80:12324)||2006|
CD46-utilizing adenoviruses inhibit C/EBPbeta-dependent expression of proinflammatory cytokines.
MA5-11222 was used in western blot to study the mechanism by which CD46-utilizing adenoviruses inhibit inflammatory cytokine expression
|Iacobelli-Martinez M,Nepomuceno RR,Connolly J,Nemerow GR||Journal of virology (79:11259)||2005|
Replication-dependent transgene expression from a conditionally replicating adenovirus via alternative splicing to a heterologous splice-acceptor site.
MA5-11222 was used in western blot to develop oncolytic adenoviruses for cancer therapy
|Carette JE,Graat HC,Schagen FH,Abou El Hassan MA,Gerritsen WR,van Beusechem VW||The journal of gene medicine (7:1053)||2005|
Hypoxia reduces adenoviral replication in cancer cells by downregulation of viral protein expression.
MA5-11222 was used in western blot to study the role of hypoxia in downregulating viral protein expression in cancer cells
|Pipiya T,Sauthoff H,Huang YQ,Chang B,Cheng J,Heitner S,Chen S,Rom WN,Hay JG||Gene therapy (12:911)||2005|
Robust infectivity and replication of Delta-24 adenovirus induce cell death in human medulloblastoma.
MA5-11222 was used in western blot to study the ability of a mutant Delta-24 adenovirus to induce cell death in human medulloblastoma
|Stolarek R,Gomez-Manzano C,Jiang H,Suttle G,Lemoine MG,Fueyo J||Cancer gene therapy (11:713)||2004|
Fiber-mosaic adenovirus as a novel approach to design genetically modified adenoviral vectors.
MA5-11222 was used in western blot to evaluate fiber-mosaic adenovirus as a novel adenoviral vector
|Pereboeva L,Komarova S,Mahasreshti PJ,Curiel DT||Virus research (105:35)||2004|
An adenovirus vector with a chimeric fiber derived from canine adenovirus type 2 displays novel tropism.
MA5-11222 was used in western blot to develop a novel chimeric adenovirus vector with broader cell infectivity
|Glasgow JN,Kremer EJ,Hemminki A,Siegal GP,Douglas JT,Curiel DT||Virology (324:103)||2004|
Conditionally replicative adenovirus expressing p53 exhibits enhanced oncolytic potency.
MA5-11222 was used in western blot to study the oncolytic potency and therapeutic potential of conditionally replicative adenovirus expressing p53
|van Beusechem VW,van den Doel PB,Grill J,Pinedo HM,Gerritsen WR||Cancer research (62:6165)||2002|
INGN 007, an oncolytic adenovirus vector, replicates in Syrian hamsters but not mice: comparison of biodistribution studies.
MA5-11222 was used in immunohistochemistry to compare the biodistribution of an oncolytic adenovirus in Syrian hamsters in which it replicates and mice in which it does not replicate
|Ying B,Toth K,Spencer JF,Meyer J,Tollefson AE,Patra D,Dhar D,Shashkova EV,Kuppuswamy M,Doronin K,Thomas MA,Zumstein LA,Wold WS,Lichtenstein DL||Cancer gene therapy (16:625)||2009|
Autophagy pathways in glioblastoma.
MA5-11222 was used in immunohistochemistry to discus the role and therapeutic potential of autophagy pathways in glioblastoma
|Jiang H,White EJ,Conrad C,Gomez-Manzano C,Fueyo J||Methods in enzymology (453:273)||2009|
Examination of the therapeutic potential of Delta-24-RGD in brain tumor stem cells: role of autophagic cell death.
MA5-11222 was used in immunohistochemistry to study the therapeutic potential of an oncolytic adenovirus in brain tumor stem cells
|Jiang H,Gomez-Manzano C,Aoki H,Alonso MM,Kondo S,McCormick F,Xu J,Kondo Y,Bekele BN,Colman H,Lang FF,Fueyo J||Journal of the National Cancer Institute (99:1410)||2007|
A human adenoviral vector with a chimeric fiber from canine adenovirus type 1 results in novel expanded tropism for cancer gene therapy.
MA5-11222 was used in immunohistochemistry to study the expanded tropism of a novel human adenoviral vector containing a chimeric fibre and the significance for cancer gene therapy
|Stoff-Khalili MA,Rivera AA,Glasgow JN,Le LP,Stoff A,Everts M,Tsuruta Y,Kawakami Y,Bauerschmitz GJ,Mathis JM,Pereboeva L,Seigal GP,Dall P,Curiel DT||Gene therapy (12:1696)||2005|
Telomerase-dependent oncolytic adenovirus for cancer treatment.
MA5-11222 was used in immunohistochemistry to develop a telomerase-dependent oncolytic adenovirus for cancer treatment
|Huang TG,Savontaus MJ,Shinozaki K,Sauter BV,Woo SL||Gene therapy (10:1241)||2003|
A ligand-pseudoreceptor system based on de novo designed peptides for the generation of adenoviral vectors with altered tropism.
MA5-11222 was used in immunocytochemistry and western blot to develop a new system for producing adenoviruses ablated of natural tropism
|Zeng Y,Pinard M,Jaime J,Bourget L,Uyen Le P,O'Connor-McCourt MD,Gilbert R,Massie B||The journal of gene medicine (10:355)||2008|