|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Tissue / cell preparation adenovirus.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||0.2-0.4 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg of protein lysate|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91861 detects Adenovirus type 5 E1A from virus samples.
MA1-91861 has been successfully used in immunofluorescence, immunohistochemistry, Western blot, and immunoprecipitation applications. Positive control of 293 cells suggested. Requires boiling tissue sections in 10 mM citrate buffer, pH 6, 10-20 minutes followed by cooling at room temperature for 20 minutes.
The MA1-91861 immunogen is tissue / cell preparation adenovirus.
Store at 4°C short term. Aliquot and store at -20°C or below. Add glycerol to a final volume of 50% for added stability. Avoid freeze/thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.