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|Tested species reactivity||Virus|
|Published species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Ad5 Hexon protein purified from mammalian cell|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
A suggested positive control for this product is Ad5-infected 293 cells.
Hexon is a one of adenovirus capsid proteins (240 hexons per particle). Each hexon capsomere is homotrimer of the hexon protein, a complex protein of over 900 residues. 49 human adenovirus serotypes have been discovered, and divided into six subgroup, A to E, based on a variety of observation. They are an important cause of respiratory illness, conjunctivitis, and gastroenteritis and have more recently been recognized as a potential cause of sexually transmitted diseases. Adenovirus vectors, particularly those based on Ad5, have shown great promise for the development of gene therapy protocols and have been used for clinical purpose. More recently, polyclonal antibodies against adenovirus were used for quantifying adenoviral titers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Systemic delivery of a breast cancer-detecting adenovirus using targeted microbubbles.
LF-MA0177 was used in flow cytometry to develop a novel method for targeting breast cancer cells using adenovirus and microbubbles
|Warram JM,Sorace AG,Saini R,Borovjagin AV,Hoyt K,Zinn KR||Cancer gene therapy (19:545)||2012|