Invitrogen

Adenylate Cyclase 3 Polyclonal Antibody

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Adenylate Cyclase 3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Adenylate Cyclase III in cultured rat neurons and glia using an Adenylate Cyclase III polyclonal antibody (Product # PA5-35382) showing strong staining of neuronal cilia (red) and axonal and dendritic staining of Alpha II Spectrin (Product # MA5-18118) (green) revealing the submembraneous cytoskeleton and DNA (blue).

Adenylate Cyclase 3 Antibody in Western Blot (WB) — Western blot analysis of Adenylate Cyclase III in rat cortex lysate using a Adenylate Cyclase III polyclonal antibody (Product # PA5-35382). Results show a band at ~160kDa.

Adenylate Cyclase 3 Antibody in Immunohistochemistry (IHC) — Figure 6. BrdU/AC3/OMP triple immunostaining at 10 and 12 d following BrdU administration. A , Confocal image at low magnification representing flattened stacks of seven to nine 1-mum-thick optical sections. BrdU + (green) OSNs coexpressing AC3 (red) and OMP (blue) at day 12 after BrdU injections (arrows). B , Pie charts quantifying BrdU + cells expressing AC3 (maroon) or OMP (gray), coexpressing AC3 and OMP (yellow), or not expressing either AC3 and/or OMP (green) at DPI-B-10 and DPI-B-12. C , D , Higher-magnification confocal images showing examples of BrdU + cells (green) coexpressing AC3 (red) and/or OMP (blue). C1-C4 , Dotted circle denotes a BrdU + cell ( C2 , green) coexpressing OMP ( C1 , blue) and AC3 ( C3 , red), while a dotted square highlights a BrdU + cell ( C2 , green) expressing OMP ( C1 , blue), but not AC3 ( C3 , red); C4 shows a merged image. D1-D4 , A dotted circle shows a BrdU + cell ( D2 , green) coexpressing OMP ( D1 , blue) and AC3 ( D3 , red); D4 shows a merged image. E1-E4 , Arrow pointing to a BrdU + cell ( E2 , green) expressing OMP ( E1 , blue), but not AC3 ( E3 ). Next to the BrdU + cell, there is a BrdU - cell (arrowhead) coexpressing OMP ( E1 , blue) and AC3 ( E3 , red); E4 shows a merged image. Dashed white lines delineate the surface of the OE (top) and basal lamina (bottom). Scale bars: A , 25 mum; C-E , 10 mum.

Product Details

PA5-35382

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:500	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to the C-terminal region of rat ADCY3 conjugated to KLH View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	~1.0 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	5mM sodium azide
Storage conditions	-20°C
RRID	AB_2552692

Product Specific Information

This antibody contains enough material to conduct 10 mini-Western blots.

Target Information

This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by Autophagy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yang D,Wu X,Wang W,Zhou Y,Wang Z

Published figure using Adenylate Cyclase 3 polyclonal antibody (Product # PA5-35382) in Western Blot

Sat Jan 01 00:00:00 EST 2022

Chemokine CCL5 promotes robust optic nerve regeneration and mediates many of the effects of CNTF gene therapy.

Proceedings of the National Academy of Sciences of the United States of America

Xie L,Yin Y,Benowitz L

PA5-35382 was used in Immunohistochemistry to investigate whether CNTF gene therapy acts indirectly through other immune mediators.

Tue Mar 02 00:00:00 EST 2021

Sequential Maturation of Olfactory Sensory Neurons in the Mature Olfactory Epithelium.

eNeuro

Liberia T,Martin-Lopez E,Meller SJ,Greer CA

Published figure using Adenylate Cyclase 3 polyclonal antibody (Product # PA5-35382) in Immunohistochemistry

Fri May 08 00:00:00 EDT 2020

Elevating Growth Factor Responsiveness and Axon Regeneration by Modulating Presynaptic Inputs.

Neuron

Zhang Y,Williams PR,Jacobi A,Wang C,Goel A,Hirano AA,Brecha NC,Kerschensteiner D,He Z

PA5-35382 was used in Immunohistochemistry to study methods to improve growth-factor responsiveness in mature neurones by over-expressing Lin28 within the retina.

Wed Jul 03 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: AC-III; AC3; Adenylate cyclase type 3; Adenylate cyclase type III; Adenylate cyclase, olfactive type; Adenylyl cyclase 3; adenylyl cyclase, type III; ATP pyrophosphate-lyase 3; type III adenylyl cyclase

Gene Aliases: AC-III; AC3; ADCY3; KIAA0511; mKIAA0511

UniProt ID: (Human) O60266, (Rat) P21932, (Mouse) Q8VHH7

Entrez Gene ID: (Human) 109, (Rat) 64508, (Mouse) 104111

Function(s)