Invitrogen

Adenylate Kinase 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (39) Adenylate Kinase 1 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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Adenylate Kinase 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Adenylate Kinase 1 in human cell line A-431 shows positivity in cytoplasm. Samples were probed using an Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-52297).

Adenylate Kinase 1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Adenylate Kinase 1 in human fallopian tube and small intestine tissues using Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-52297). Corresponding AK1 RNA-seq data are presented for the same tissues.

Adenylate Kinase 1 Antibody in Western Blot (WB) — Western blot analysis of Adenylate Kinase 1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-52297).

Adenylate Kinase 1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Adenylate Kinase 1 demonstrates antibody specificity. Immunohistochemical analysis of Adenylate Kinase 1 using anti-Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-52297), shows significant staining of Adenylate Kinase 1 in human fallopian tube and shows minimal or weak staining in human small intestine tissues. The relative expression levels of Adenylate Kinase 1 within each tissue is shown using RNA-Seq.

Product Details

PA5-52297

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Adenylate Kinase 1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2637699

Product Specific Information

Immunogen sequence: EKGQLVPLET VLDMLRDAMV AKVNTSKGFL IDGYPREVQQ GEEFERRIGQ PTLLLYVDAG PETMTQRLLK RGETSGRVDD NEETIKKRLE TYYKATEPVI AFYEKRGIVR KVNAEGS

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 69%.

Target Information

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Adenylate kinase isoenzyme 1; adenylate kinase isoenzyme 1 {ECO:0000255|HAMAP-Rule:MF_03171}; Adenylate monophosphate kinase; adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03171}; AK 1; AK 1 {ECO:0000255|HAMAP-Rule:MF_03171}; ATP-AMP transphosphorylase 1; ATP-AMP transphosphorylase 1 {ECO:0000255|HAMAP-Rule:MF_03171}; ATP:AMP phosphotransferase; ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03171}; cytosolic adenylate kinase; Myokinase; myokinase {ECO:0000255|HAMAP-Rule:MF_03171}; testis secretory sperm binding protein Li 58j

Gene Aliases: Ak 1; Ak-1; AK1; B430205N08Rik; HTL-S-58j

UniProt ID: (Human) P00568, (Mouse) Q9R0Y5, (Rat) Q5EBC5

Entrez Gene ID: (Human) 203, (Mouse) 11636, (Rat) 24183

Molecular Function: nucleotide kinase