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Immunofluorescent analysis of Adenylate kinase 2 showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using an Adenylate kinase 2 polyclonal antibody (Product # PA5-28611) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 239 of Human Adenylate kinase 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28611 targets Adenylate kinase 2 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-28611 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 239 of Human Adenylate kinase 2.
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
adenylate kinase 2; adenylate kinase 2, mitochondrial; adenylate kinase isoenzyme 2; adenylate kinase isoenzyme 2, mitochondrial; adenylate monophosphate kinase; ADK2; AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; mitochondrial; testis secretory sperm-binding protein Li 220n
ADK2; Ak-2; AK2; D4Ertd220e