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Invitrogen
Adipolean (gAcrp30) Polyclonal Antibody, Biotin, PeproTech®
FIGURE: 1 / 1
Adipolean (gAcrp30) Antibody (500-P193GBT-1MG) in ELISA
Sandwich ELISA: To detect hAdipolean by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Adipolean (gAcrp30) Polyclonal Antibody, Biotin (Product # 500-P193GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Adipolean (gAcrp30) Polyclonal Antibody (Product # 500-P193G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean.
Product Details
500-P193GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human gAcrp30/Adipolean
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929764
Product Specific Information
AA Sequence of recombinant protein: MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human gAcrp30/Adipolean. Anti-Human gAcrp30/Adipolean-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hAdipolean by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean (500-P193G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean.
Western Blot: To detect Human Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P193GBT-1MG will be provided as 2 x 500 µg
Target Information
gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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