Immunofluorescence analysis of Adiponectin was performed using 70% confluent 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10065-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adiponectin (19F1) Mouse Monoclonal Antibody (Product # MA1-054) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rabbit|
|Published species reactivity||Rabbit, Human, Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full length, recombinant, human adiponectin.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2-4 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-054 detects adiponectin from human, rabbit and mouse samples.
MA1-054 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~30 kDa protein representing Adiponectin in 3T3-L1 adipocytes. Immunofluorescence and ELISA of Adiponectin can also be performed using MA1-054.
The MA1-054 immunogen is full length, recombinant, human adiponectin.
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expansion of Bone Marrow Adipose Tissue During Caloric Restriction Is Associated With Increased Circulating Glucocorticoids and Not With Hypoleptinemia.
MA1-054 was used in western blot to test if caloric restriction affects adiponectin production by bone marrow adipose tissue
|Cawthorn WP,Scheller EL,Parlee SD,Pham HA,Learman BS,Redshaw CM,Sulston RJ,Burr AA,Das AK,Simon BR,Mori H,Bree AJ,Schell B,Krishnan V,MacDougald OA||Endocrinology (157:508)||2016|
Effect of 12 Weeks of Periodized Resistance Training Upon Total Plasma Adiponectin Concentration in Healthy Young Men.
MA1-054 was used in western blot to determine the effect of periodized resistance training on plasma adiponectin
|Davis GR,Stephens JM,Nelson AG||Journal of strength and conditioning research (29:3097)||2015|
St. John's Wort Has Metabolically Favorable Effects on Adipocytes In Vivo.
MA1-054 was used in western blot to study the effects of St John's Wart on adipocyte function in vivo
|Fuller S,Richard AJ,Ribnicky DM,Beyl R,Mynatt R,Stephens JM||Evidence-based complementary and alternative medicine : eCAM (2014:null)||2014|
Naringenin inhibits adipogenesis and reduces insulin sensitivity and adiponectin expression in adipocytes.
MA1-054 was used in western blot to study the inhibition of adipogenesis and the decreased insulin sensitivity and adiponectin expression of adipocytes treated with naringenin
|Richard AJ,Amini-Vaughan Z,Ribnicky DM,Stephens JM||Evidence-based complementary and alternative medicine : eCAM (2013:null)||2013|
Biochemical changes induced by strontium ranelate in differentiating adipocytes.
MA1-054 was used in western blot to study the effect of the osteoporosis therapeutic strontium ranelate on differentiating adipocytes
|Vidal C,Gunaratnam K,Tong J,Duque G||Biochimie (95:793)||2013|
The transcription factor paired-related homeobox 1 (Prrx1) inhibits adipogenesis by activating transforming growth factor-ß (TGFß) signaling.
MA1-054 was used in western blot to study the role of TGF-beta in the mechanism by which Prrx1 inhibits adipogenesis
|Du B,Cawthorn WP,Su A,Doucette CR,Yao Y,Hemati N,Kampert S,McCoin C,Broome DT,Rosen CJ,Yang G,MacDougald OA||The Journal of biological chemistry (288:3036)||2013|
Snail, a transcriptional regulator, represses adiponectin expression by directly binding to an E-box motif in the promoter.
MA1-054 was used in western blot to investigate the mechanism for the repressive effect of Snail on adiponectin expression
|Park YM,Lee YH,Kim SH,Lee EY,Kim KS,Williams DR,Lee HC||Metabolism: clinical and experimental (61:1622)||2012|
HIV protease inhibitors activate the adipocyte renin angiotensin system.
MA1-054 was used in western blot to investigate the effect of HIV protease inhibitors on the activation of adipose renin angiotensin system
|Boccara F,Auclair M,Cohen A,Lefèvre C,Prot M,Bastard JP,Capeau J,Caron-Debarle M||Antiviral therapy (15:363)||2010|
LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice.
MA1-054 was used in immunohistochemistry to study the effect of LTP induction on cortactin translocation in wild-type and fmr1 knock-out mice
|Seese RR,Babayan AH,Katz AM,Cox CD,Lauterborn JC,Lynch G,Gall CM||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:7403)||2012|