|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||E.coli expressed recombinant full length protein (Rat).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 0.5% BSA, 30% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29901 detects Adiponectin from rat, mouse, human samples.
PA1-29901 has been successfully used in immunoprecipitation, and Western blot applications.
The PA1-29901 immunogen is: E.coli expressed recombinant full length protein (Rat).
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also know as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced > 100 fold. Adiponmectin imporves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3k) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is signigicantly reduced in adipose tissue of obese patients with type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.