This antibody is predicted to react with human, rat, chimpanzee, Rhesus monkey, canine, bovine and chicken based on sequence homology. Boiling samples prior to Western blot is not recommended.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
AF-6, also known as afadin, is expressed in a variety of cell types. At least 5 isoforms of this protein are produced by alternative splicing and the 'canonical' sequence is designated as l-afadin. Chromosomal aberration of the MLLT4 gene and tight junctions and Notch signaling pathways cross-communication. This happens through physical interaction with Ras, Notch and Dishevelled, a key Wingless effector. AF-6 also controls integrin-mediated cell adhesion. This is done at least partially by regulating Rap1 activation through recruitment of both SPA-1 and Rap1GTP. These results demonstrate that AF-6 is an important molecule mediating cell-cell adhesion but is also capable of modulating several key signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Afadin; Afadin adherens junction formation factor; MLLT4; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; Protein Af-6
Gene Aliases: 5033403D15Rik; Af-6; AF6; Afadin; Afdn; Gm314; I-afadin; Mllt4; S-afadin
UniProt ID: (Mouse) Q9QZQ1
Entrez Gene ID: (Mouse) 17356