This antibody is predicted to react with human, rat, chimpanzee, Rhesus monkey, canine, bovine and chicken based on sequence homology. Boiling samples prior to Western blot is not recommended.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Afadin; Afadin adherens junction formation factor; FLJ34371; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; Protein Af-6; RP3-431P23.3; RP3-470B24.4
Gene Aliases: 5033403D15Rik; Af-6; AF6; Afadin; Afdn; Gm314; I-afadin; Mllt4; S-afadin
UniProt ID: (Mouse) Q9QZQ1
Entrez Gene ID: (Mouse) 17356