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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human natural aggrecan (proteoglycan), purified from human articular cartilage.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
|Western Blot (WB)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|ELISA (ELISA)||See 2 publications below|
This product is recommended as a detection antibody in Sandwich ELISA applications.
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (~ 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Translational development of an ADAMTS-5 antibody for osteoarthritis disease modification.
AHP0022 was used in blocking or activating experiment to assess the therapeutic use of monoclonal antibodies to ADAMTS-5 and ADAMTS-4 in pathogenic cartilage degradation
|Larkin J,Lohr TA,Elefante L,Shearin J,Matico R,Su JL,Xue Y,Liu F,Genell C,Miller RE,Tran PB,Malfait AM,Maier CC,Matheny CJ||Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society (23:1254)||2015|
Poly(γ-Glutamic Acid) as an Exogenous Promoter of Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells.
AHP0022 was used in immunohistochemistry - paraffin section to examine the ability of gamma-PGA to support production of cartilaginous extracellular matrix.
|Antunes JC,Tsaryk R,Gonçalves RM,Pereira CL,Landes C,Brochhausen C,Ghanaati S,Barbosa MA,Kirkpatrick CJ||Tissue engineering. Part A (21:1869)||2015|
Inflammatory cytokines and biomarkers of cartilage metabolism 8 years after anterior cruciate ligament reconstruction: results from operated and contralateral knees.
AHP0022 was used in immunocytochemistry to discuss the role of inflammation in posttraumatic arthritis
|Åhlén M,Roshani L,Lidén M,Struglics A,Rostgård-Christensen L,Kartus J||The American journal of sports medicine (43:1460)||2015|
Hyaluronic acid enhances the mechanical properties of tissue-engineered cartilage constructs.
AHP0022 was used in immunohistochemistry - frozen section to study the mechanical properties of tissue-engineered cartilage constructs using in vitro culture models incorporating human chondrocytes from osteoarthritis patients
|Levett PA,Hutmacher DW,Malda J,Klein TJ||PloS one (9:null)||2014|
Physiological testosterone levels enhance chondrogenic extracellular matrix synthesis by male intervertebral disc cells in vitro, but not by mesenchymal stem cells.
AHP0022 was used in immunohistochemistry - frozen section to study the effect of testosterone on intervertebral disc.
|Bertolo A,Baur M,Aebli N,Ferguson SJ,Stoyanov J||The spine journal : official journal of the North American Spine Society (14:455)||2014|
Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins.
AHP0022 was used in western blot to study the association between disc degeneration and autoimmunity
|Capossela S,Schläfli P,Bertolo A,Janner T,Stadler BM,Pötzel T,Baur M,Stoyanov JV||European cells & materials (27:251)||2014|
Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis.
AHP0022 was used in western blot to study the association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis.
|Larsson S,Englund M,Struglics A,Lohmander LS||Arthritis research & therapy (12:null)||2011|
An ARGS-aggrecan assay for analysis in blood and synovial fluid.
AHP0022 was used in immunoprecipitation to evaludate a novel ARGS-aggrecan assay.
|Larsson S,Lohmander LS,Struglics A||Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society (22:242)||2014|
Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis)--a cross-sectional analysis.
AHP0022 was used in ELISA to determine the concentrations of cartilage and bone markers, and pro-inflammatory cytokines in synovial fluid collected from acutely injured knees with hemarthrosis and healthy controls.
|Swärd P,Frobell R,Englund M,Roos H,Struglics A||Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society (20:1302)||2012|
Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254).
AHP0022 was used in ELISA to assess changes after acute injury to the anterior cruciate ligament using synovial fluid and serum biomarkers.
|Catterall JB,Stabler TV,Flannery CR,Kraus VB||Arthritis research & therapy (12:null)||2011|
AGC1; AGCAN; aggrecan; aggrecan 1; aggrecan core protein; cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPGCP; large aggregating proteoglycan; MSK16; SEDK
AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK