|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human natural aggrecan (proteoglycan), purified from human articular cartilage.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 3 publications below|
|Western Blot (WB)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|ELISA (ELISA)||See 5 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
This product is recommended as a detection antibody in Sandwich ELISA applications.
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (~ 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Translational development of an ADAMTS-5 antibody for osteoarthritis disease modification.
AHP0022 was used in blocking or activating experiment to assess the therapeutic use of monoclonal antibodies to ADAMTS-5 and ADAMTS-4 in pathogenic cartilage degradation
|Larkin J,Lohr TA,Elefante L,Shearin J,Matico R,Su JL,Xue Y,Liu F,Genell C,Miller RE,Tran PB,Malfait AM,Maier CC,Matheny CJ||Osteoarthritis and cartilage (23:1254)||2015|
Poly(¿-Glutamic Acid) as an Exogenous Promoter of Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells.
AHP0022 was used in immunohistochemistry - paraffin section to examine the ability of gamma-PGA to support production of cartilaginous extracellular matrix.
|Antunes JC,Tsaryk R,Gonçalves RM,Pereira CL,Landes C,Brochhausen C,Ghanaati S,Barbosa MA,Kirkpatrick CJ||Tissue engineering. Part A (21:1869)||2015|
|Not Applicable||Not Cited||
Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels.
AHP0022 was used in immunohistochemistry - paraffin section to study how mesencymal stem cells in alginate hydrogels can form neocartilage in regards to biochemical and structural characterization
|Olderøy MØ,Lilledahl MB,Beckwith MS,Melvik JE,Reinholt F,Sikorski P,Brinchmann JE||PloS one (9:null)||2014|
The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus.
AHP0022 was used in immunohistochemistry - paraffin section to compare Histochoice and formalin fixation for cartilaginous tissues
|Melrose J,Smith SM,Smith MM,Little CB||Biotechnic and histochemistry : official publication of the Biological Stain Commission (83:47)||2008|
Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint.
AHP0022 was used in immunohistochemistry - paraffin section to study perlecan localization in the articular and epiphyseal growth plate cartilages of stifle joints in pedigree merino sheep
|Melrose J,Smith S,Cake M,Read R,Whitelock J||Histochemistry and cell biology (123:561)||2005|
Inflammatory cytokines and biomarkers of cartilage metabolism 8 years after anterior cruciate ligament reconstruction: results from operated and contralateral knees.
AHP0022 was used in immunocytochemistry to discuss the role of inflammation in posttraumatic arthritis
|Åhlén M,Roshani L,Lidén M,Struglics A,Rostgård-Christensen L,Kartus J||The American journal of sports medicine (43:1460)||2015|
|Not Applicable||2.28 µg/ml||
Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage.
AHP0022 was used in immunocytochemistry to test if osteoarthritis chondrocytes are suitable for tissue engineering of articular cartilage.
|Fernandes AM,Herlofsen SR,Karlsen TA,Küchler AM,Fløisand Y,Brinchmann JE||PloS one (8:null)||2013|
The importance of connexin hemichannels during chondroprogenitor cell differentiation in hydrogel versus microtissue culture models.
AHP0022 was used in immunocytochemistry to examine the influence of gap junction-mediated cell-cell communication on chondrogenesis of bone marrow-derived mesenchymal stromal cells and articular chondrocytes.
|Schrobback K,Klein TJ,Woodfield TB||Tissue engineering. Part A (21:1785)||2015|
The cartilage matrix molecule components produced by human foetal cartilage rudiment cells within scaffolds and the role of exogenous growth factors.
AHP0022 was used in immunohistochemistry (paraffin) to generate a three-dimensional cartilage scaffold and incorporate fetal cartilage rudiment cells.
|Chuang CY,Shahin K,Lord MS,Melrose J,Doran PM,Whitelock JM||Biomaterials (33:4078)||2012|
Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker.
AHP0022 was used in immunohistochemistry to determine the localization of type II collagen, aggrecan, and perlecan in young ovine joints
|Smith SM,Shu C,Melrose J||Histochemistry and cell biology (134:251)||2010|
Culture of chondrocytes in alginate surrounded by fibrin gel: characteristics of the cells over a period of eight weeks.
AHP0022 was used in immunocytochemistry to describe methods to generate cartilage from human articular chondrocytes.
|Almqvist KF,Wang L,Wang J,Baeten D,Cornelissen M,Verdonk R,Veys EM,Verbruggen G||Annals of the rheumatic diseases (60:781)||2001|
Hyaluronic acid enhances the mechanical properties of tissue-engineered cartilage constructs.
AHP0022 was used in immunohistochemistry - frozen section to study the mechanical properties of tissue-engineered cartilage constructs using in vitro culture models incorporating human chondrocytes from osteoarthritis patients
|Levett PA,Hutmacher DW,Malda J,Klein TJ||PloS one (9:null)||2014|
Physiological testosterone levels enhance chondrogenic extracellular matrix synthesis by male intervertebral disc cells in vitro, but not by mesenchymal stem cells.
AHP0022 was used in immunohistochemistry - frozen section to study the effect of testosterone on intervertebral disc.
|Bertolo A,Baur M,Aebli N,Ferguson SJ,Stoyanov J||The spine journal : official journal of the North American Spine Society (14:455)||2014|
|Not Applicable||2 µg/ml||
Silkworm and spider silk scaffolds for chondrocyte support.
AHP0022 was used in immunohistochemistry - frozen section to create scaffolds with silkworm cocoon, spider egg sac, and spider dragline silk fibers and examine their use for chondrocyte attachment and support
|Gellynck K,Verdonk PC,Van Nimmen E,Almqvist KF,Gheysens T,Schoukens G,Van Langenhove L,Kiekens P,Mertens J,Verbruggen G||Journal of materials science. Materials in medicine (19:3399)||2008|
Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins.
AHP0022 was used in western blot to study the association between disc degeneration and autoimmunity
|Capossela S,Schläfli P,Bertolo A,Janner T,Stadler BM,Pötzel T,Baur M,Stoyanov JV||European cells and materials (27:251)||2014|
Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis.
AHP0022 was used in western blot to study the association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis.
|Larsson S,Englund M,Struglics A,Lohmander LS||Arthritis research and therapy (12:null)||2011|
An ARGS-aggrecan assay for analysis in blood and synovial fluid.
AHP0022 was used in immunoprecipitation to evaludate a novel ARGS-aggrecan assay.
|Larsson S,Lohmander LS,Struglics A||Osteoarthritis and cartilage (22:242)||2014|
Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis)--a cross-sectional analysis.
AHP0022 was used in ELISA to determine the concentrations of cartilage and bone markers, and pro-inflammatory cytokines in synovial fluid collected from acutely injured knees with hemarthrosis and healthy controls.
|Swärd P,Frobell R,Englund M,Roos H,Struglics A||Osteoarthritis and cartilage (20:1302)||2012|
The association between changes in synovial fluid levels of ARGS-aggrecan fragments, progression of radiographic osteoarthritis and self-reported outcomes: a cohort study.
AHP0022 was used in ELISA to examine if changes in aggrecanase generated ARGS-aggrecan in synovial fluid is associated with progression of radiographic knee osteoarthritis and patient-reported outcome.
|Larsson S,Englund M,Struglics A,Lohmander LS||Osteoarthritis and cartilage (20:388)||2012|
Not all lubricin isoforms are substituted with a glycosaminoglycan chain.
AHP0022 was used in ELISA to determine the structure and function of the glycosaminoglycan chain of lubricin in human synovial fluid.
|Lord MS,Estrella RP,Chuang CY,Youssef P,Karlsson NG,Flannery CR,Whitelock JM||Connective tissue research (53:132)||2012|
Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254).
AHP0022 was used in ELISA to assess changes after acute injury to the anterior cruciate ligament using synovial fluid and serum biomarkers.
|Catterall JB,Stabler TV,Flannery CR,Kraus VB||Arthritis research and therapy (12:null)||2011|
Development of a novel clinical biomarker assay to detect and quantify aggrecanase-generated aggrecan fragments in human synovial fluid, serum and urine.
AHP0022 was used in ELISA to describe a method to detect aggrecanase-cleaved fragments of aggrecan in human serum and urine and use this assay to screen for inhibitors
|Swearingen CA,Carpenter JW,Siegel R,Brittain IJ,Dotzlaf J,Durham TB,Toth JL,Laska DA,Marimuthu J,Liu C,Brown DP,Carter QL,Wiley MR,Duffin KL,Mitchell PG,Thirunavukkarasu K||Osteoarthritis and cartilage (18:1150)||2010|
|Not Applicable||Not Cited||
The structure, location, and function of perlecan, a prominent pericellular proteoglycan of fetal, postnatal, and mature hyaline cartilages.
AHP0022 was used in immunohistochemistry to study the localization and function of perlecan in human fetal, postnatal, and mature hyaline cartilages
|Melrose J,Roughley P,Knox S,Smith S,Lord M,Whitelock J||The Journal of biological chemistry (281:36905)||2006|
|Not Applicable||Not Cited||
Characterisation of human knee meniscus cell phenotype.
AHP0022 was used in flow cytometry to examine the extracellular matrix of human meniscus cells cultured under different conditions
|Verdonk PC,Forsyth RG,Wang J,Almqvist KF,Verdonk R,Veys EM,Verbruggen G||Osteoarthritis and cartilage (13:548)||2005|