Invitrogen

Aggrecan Monoclonal Antibody (BC-3)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

24 Published Figures

37 References

View all (16) Aggrecan antibodies

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Aggrecan Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Aggrecan was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Aggrecan (BC-3) Mouse Monoclonal Antibody (Product # MA3-16888) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the extracellular matrix. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Aggrecan Antibody in Western Blot (WB) — Western blot analysis of Aggrecan neoepitope fragments, generated as a result of Aggrecanase proteolytic activity, was performed using cleaved and uncleaved recombinant Interglobular domain (IGD) of Aggrecan (Product # RP-77527), followed by detection with an Aggrecan monoclonal antibody (Product # MA3-16888). IGD (5 µg) was incubated for the indicated times with or without 1U recombinant Aggrecanase. IGD samples and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) were loaded onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and full length IGD, as well as cleaved Aggrecan-IGD fragments were visualized (bottom panel) using a Reversible Protein Stain Kit for PVDF Membranes (Product # 24585). For specific neo-epitope fragment detection (top panel), the membrane was incubated in Destain solution (included in the kit), blocked with 5% BSA/TBST for 1 hour, and probed with an Aggrecan monoclonal antibody (Product # MA3-16888) at a dilution of 1:1000 overnight at 4°C on a rocking platform. The membrane was washed in TBS-0.1% Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Aggrecan Antibody in ELISA (ELISA) — ELISA analysis of Aggrecan was performed by coating Amine-binding Maleic Anhydride Activated Plates (Product # 15110) overnight with 100 µL of 2 µg/mL Aggrecan Interglobular Domain recombinant protein (Product # RP-77527). Plates were blocked for 1 hour at room temperature with StartingBlock Blocking Buffer (Product # 37538) and incubated with 100 µL per well of an Aggrecan monoclonal antibody (Product # MA3-16888) in duplicate at 25, 12.5, 6.25, 3.125, 1.563, 0.78 µg/100 µL with serial dilutions of for 2 hours at room temperature. The plate was washed and incubated with 100 µL per well of goat anti-mouse IgG-HRP secondary antibody (Product # 31430) in all test wells at 1:30,000 for 1 hour at room temperature. Detection was performed using 1-Step Ultra TMB - ELISA Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbance values were read on a spectrophotometer at 450-550 nm.

Aggrecan Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 3 circPhc3 is involved in the secretion of extracellular matrix and cartilage-degrading enzymes. (A) circPhc3 expression levels in chondrocytes transfected with shRNA-NC or shRNA-circPhc3 were analysed by RT-qPCR. The results showed that circPhc3 silencing was effective. ** P<0.01 (n=4). (B) circPhc3 expression levels in chondrocytes transfected with Mock and circPhc3 overexpression vector were analysed by RT-qPCR. The results showed that circPhc3 overexpression was effective. ** P<0.01 (n=4). Protein expression levels were (C) determined by western blotting and semi-quantified for (D) MMP-3, (E) Aggrecan and (F) Col2alpha1 in chondrocytes transfected with shRNA-NC, shRNA-circPhc3, Mock or circPhc3 overexpression vector. ** P<0.01 (n=4). mRNA expression levels of (G) MMP-3, (H) Aggrecan and (I) Col2alpha1 in chondrocytes transfected with shRNA-NC, shRNA-circPhc3, Mock or circPhc3 overexpression vector were detected by RT-qPCR. * P<0.05 and ** P<0.01 (n=4). (J) Col2alpha1, Aggrecan and MMP-3 expression in chondrocytes transfected with shRNA-NC, shRNA-circPhc3, Mock and circPhc3 overexpression vector were detected by immunofluorescence staining (n=4). circ, circular RNA; shRNA, short hairpin RNA; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; MMP, matrix metalloproteinase; Col2alpha1, collagen type II, alpha1.

Aggrecan Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (WB, ICC/IF, IHC) — Fig. 1 The extracellular matrix expression and cholesterol efflux rate decreases in osteoarthritis (OA) chondrocytes. a) and b) Western blot analysis of aggrecan, collagen II, and peroxisome proliferator-activated receptor (PPARgamma) expression in chondrocytes. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the endogenous control. c) The difference in cholesterol efflux rate between the control and osteoarthritis (OA) groups. d) and e) Representative immunofluorescence staining and quantification of the positive cell numbers. Chondrocytes are stained blue and the target proteins are stained red (scale bar = 100 um). DAPI, 4',6-diamidino-2-phenylindole. All p-values calculated using independent-samples t -test.

Aggrecan Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Fig 6 Reduced Acan contributes to Hapln1a knockdown phenotypes. (A) Longitudinal section of fin rays treated with standard control morpholino (Standard control MO) and acanb ATG-blocking morpholino (Acanb MO1) or splice blocking morpholino (Acanb MO2). Immuno-staining for Acan (red) and counterstained for nuclei with To-pro (blue). Compared to the standard MO treated fins, Acanb MO treated fins show reduced staining for Acan. White arrow identifies the basal layer of epithelium; yellow arrow identifies Acan staining in the lepidotrichia, arrow head identifies the bone; m, mesenchyme; e, epithelium. Scale bar represents 20 mum. (B) Bar graph shows that regenerate length, segment length and cell proliferation are significantly reduced upon acanb knockdown using both MO1 and MO2. The mean of percent similarity for the MO treated experimental group and the corresponding control group were estimated and compared. Statistical significance was determined using the student's t -test (P<0.05) and the error bars represent standard error of mean. The hashed bars indicate the extent of hapln1a knockdown effect compared to acanb knockdown. (C) Representative alizarin red stained fins showing extent of bone calcification. The black line indicates the amputation plane. (D) The extent of mineralization was calculated as the ratio of the zone of mineralization (extent of detectable alizarin red staining length) to the total regenerate length. The mean of percent similarity for the MO tre

Aggrecan Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunohistochemistry (Frozen)

Aggrecan Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 10 Chondrogenic differentiation of hMSCs cultured for 21 days on scaffolds assessed by A immunostaining of aggrecan and collagen II expression, and B western blot analysis of aggrecan, collagen II, ERK and pERK expression (n = 2). The relative expression values are given as the fold change compared to PUS/GM group. *p < 0.05, **p < 0.01 and ***p < 0.001

Aggrecan Antibody in Flow Cytometry (Flow) — Figure 2 Changes in the ECM composition of NHDF after a three-day RPM-exposure: Immunofluorescence images of 1 g -control cells, RPM-AD and RPM-MCS of laminin ( A - C ) and fibronectin ( D - F ). Transcriptional and translational laminin analysis: Quantitative gene expression levels of LAMA3 ( G ), intracellular laminin levels ( H ) and flow cytometric analysis of laminin-labeled cells ( I ) displaying the percentage of laminin-positive cells as well as alteration of the median fluorescence intensity (MFI). Transcriptional and translational fibronectin analysis: Quantitative gene expression levels of FN1 ( J ), intracellular fibronectin levels ( K ) and flow cytometric analysis of fibronectin-labeled cells ( L ). Transcriptional and translational aggrecan analysis: Quantitative gene expression level of ACAN ( M ), intracellular aggrecan levels ( N ) and flow cytometric analysis of chondroitin sulfate-labeled cells ( O ). Transcriptional and translational osteopontin analysis: Quantitative gene expression levels of SPP1 ( P ), intracellular osteopontin levels ( Q ) and flow cytometric analysis of osteopontin-labeled cells ( R ). Full-length blots of cropped Western blot images are presented in Supplementary Fig. S1 . *p < 0.05 1 g vs. RPM; # p < 0.05 AD vs. MCS. Scale bars: 50 um.

Aggrecan Antibody — Fig 5 Morpholino mediated knockdown of hapln1a results in reduced Acan protein levels. Longitudinal section of fin rays treated with hapln1a control morpholino (Hapln1a MM) and hapln1a targeting morpholino (Hapln1a MO). Immuno-staining for Acan (blue) and counterstained for nuclei with Propidium Iodide (PI, red). Compared to the control Hapln1a MM treated fins, Hapln1a MO treated fins show reduced staining for Acan. Arrow identifies the basal layer of epithelium; arrow head identifies the bone; m, mesenchyme; e, epithelium. Arrow identifies the basal layer of epithelium; arrow head identifies the bone; m, mesenchyme; e, epithelium. Scale bar represents 20 mum. {TM}

Product Details

MA3-16888

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 17 publications 17 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:2,000	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	1:10-1:2,000	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	2 µg/mL	View 7 publications 7 publications
ELISA (ELISA)	1:1,000-1:5,000	-
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Dog, Horse, Cat, Guinea pig, Human, Sheep, Pig, Rabbit, Rat
Published species	Bacteria, Human, Mouse, Rat, Zebrafish
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	BC-3
Immunogen	Synthetic peptide corresponding to human aggrecan ARGSV neoepitope.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1mg/mL BSA, 30% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_568440

Product Specific Information

Purified recombinant Aggrecan Interglobular Domain (IGD) protein (Amino acids T331-G458 with C-term His-tag, expressed in E. coli), was used to validate this antibody. The recombinant protein contains cleavage sites for aggrecanases (E373-A374 in aggrecan) and matrix metalloproteinases (N341-F342 in aggrecan). The recombinant protein was cleaved in vitro using recombinant Aggrecanase. Degradation of IGD and accumulation of cleaved fragments was visualized via MEMCode reversible protein staining, followed by Western Blot using the BC-3 antibody for the specific detection of the aggrecan neoepitope. The antibody will also recognize full length IGD, as shown by ELISA, however with significantly lower affinity.

MA3-16888 has successfully been used in ELISA, immunohistochemistry (frozen and FFPE) and Western blot applications. This antibody may be used to immunostain formalin-fixed, paraffin-embedded sections, as well as alcohol-fixed frozen sections and un-fixed snap frozen sections. Samples must be deglycosylated using 0.01 Units Chondroitinase ABC, 0.01 Units Keratanase and 0.0001 Units Keratanase II per 10 µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition.

MA3-16888 detects aggrecan in human, bovine, feline, canine, guinea pig, equine, porcine, rabbit, rat, and sheep samples.

Target Information

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulation of miR-204 Expression during Chondrogenesis.

International journal of molecular sciences

Dalle Carbonare L,Bertacco J,Minoia A,Cominacini M,Bhandary L,Elia R,Gambaro G,Mottes M,Valenti MT

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Western Blot

Tue Feb 15 00:00:00 EST 2022

circPhc3 sponging microRNA‑93‑3p is involved in the regulation of chondrocyte function by mechanical instability in osteoarthritis.

International journal of molecular medicine

Wang Z,Rao Z,Wang X,Jiang C,Yang Y

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Sat Jan 01 00:00:00 EST 2022

circPhc3 sponging microRNA‑93‑3p is involved in the regulation of chondrocyte function by mechanical instability in osteoarthritis.

International journal of molecular medicine

Wang Z,Rao Z,Wang X,Jiang C,Yang Y

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Sat Jan 01 00:00:00 EST 2022

Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration.

Journal of nanobiotechnology

Deng X,Chen X,Geng F,Tang X,Li Z,Zhang J,Wang Y,Wang F,Zheng N,Wang P,Yu X,Hou S,Zhang W

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Thu Dec 02 00:00:00 EST 2021

Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration.

Journal of nanobiotechnology

Deng X,Chen X,Geng F,Tang X,Li Z,Zhang J,Wang Y,Wang F,Zheng N,Wang P,Yu X,Hou S,Zhang W

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Thu Dec 02 00:00:00 EST 2021

Post-translational regulation and proteolytic activity of the metalloproteinase ADAMTS8.

The Journal of biological chemistry

Santamaria S,Martin DR,Dong X,Yamamoto K,Apte SS,Ahnström J

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Western Blot

Mon Nov 01 00:00:00 EDT 2021

Ultrasound-targeted simvastatin-loaded microbubble destruction promotes OA cartilage repair by modulating the cholesterol efflux pathway mediated by PPARγ in rabbits.

Bone & joint research

Wang X,Wang D,Xia P,Cheng K,Wang Q,Wang X,Lin Q,Song J,Chen A,Li X

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

Ultrasound-targeted simvastatin-loaded microbubble destruction promotes OA cartilage repair by modulating the cholesterol efflux pathway mediated by PPARγ in rabbits.

Bone & joint research

Wang X,Wang D,Xia P,Cheng K,Wang Q,Wang X,Lin Q,Song J,Chen A,Li X

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

Ultrasound-targeted simvastatin-loaded microbubble destruction promotes OA cartilage repair by modulating the cholesterol efflux pathway mediated by PPARγ in rabbits.

Bone & joint research

Wang X,Wang D,Xia P,Cheng K,Wang Q,Wang X,Lin Q,Song J,Chen A,Li X

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells.

Journal of cellular physiology

Lin J,Du J,Wu X,Xu C,Liu J,Jiang L,Cheng X,Ge G,Chen L,Pang Q,Geng D,Mao H

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Western Blot

Wed Sep 01 00:00:00 EDT 2021

Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.

Materials science & engineering. C, Materials for biological applications

Riveiro A,Amorim S,Solanki A,Costa DS,Pires RA,Quintero F,Del Val J,Comesaña R,Badaoui A,Lusquiños F,Maçon ALB,Tallia F,Jones JR,Reis RL,Pou J

MA3-16888 was used in Western Blotting to show that hydrogel composite shows potential application in regeneration of subchondral defects.

Thu Jul 01 00:00:00 EDT 2021

Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes.

Frontiers in pharmacology

Zhang M,Zhang R,Zheng T,Chen Z,Ji G,Peng F,Wang W

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Western Blot

Sun May 23 00:00:00 EDT 2021

In vitro temporal HIF-mediated deposition of osteochondrogenic matrix governed by hypoxia and osteogenic factors synergy.

Journal of cellular physiology

Calejo I,Costa-Almeida R,Reis RL,Gomes ME

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Sat May 01 00:00:00 EDT 2021

Exosite inhibition of ADAMTS-5 by a glycoconjugated arylsulfonamide.

Scientific reports

Santamaria S,Cuffaro D,Nuti E,Ciccone L,Tuccinardi T,Liva F,D'Andrea F,de Groot R,Rossello A,Ahnström J

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Western Blot

Wed Jan 13 00:00:00 EST 2021

Exosite inhibition of ADAMTS-5 by a glycoconjugated arylsulfonamide.

Scientific reports

Santamaria S,Cuffaro D,Nuti E,Ciccone L,Tuccinardi T,Liva F,D'Andrea F,de Groot R,Rossello A,Ahnström J

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Western Blot

Wed Jan 13 00:00:00 EST 2021

Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.

Cell research

Wu J,Song D,Li Z,Guo B,Xiao Y,Liu W,Liang L,Feng C,Gao T,Chen Y,Li Y,Wang Z,Wen J,Yang S,Liu P,Wang L,Wang Y,Peng L,Stacey GN,Hu Z,Feng G,Li W,Huo Y,Jin R,Shyh-Chang N,Zhou Q,Wang L,Hu B,Dai H,Hao J

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Tue Sep 01 00:00:00 EDT 2020

Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.

American journal of respiratory cell and molecular biology

Jandl K,Marsh LM,Hoffmann J,Mutgan AC,Baum O,Bloch W,Thekkekara-Puthenparampil H,Kolb D,Sinn K,Klepetko W,Heinemann A,Olschewski A,Olschewski H,Kwapiszewska G

MA3-16888 was used in Immunohistochemistry to show the importance of a well-maintained BM homeostasis and suggest an active involvement of the BM in IPAH pathogenesis.

Wed Jul 01 00:00:00 EDT 2020

Silencing of long‑chain non‑coding RNA GAS5 in osteoarthritic chondrocytes is mediated by targeting the miR‑34a/Bcl‑2 axis.

Molecular medicine reports

Ji Q,Qiao X,Liu Y,Wang D,Yan J

MA3-16888 was used in Western Blot to indicate that GAS5 participated in the development of OA by regulating the biological behavior of chondrocytes via the miR‚34a/Bcl‚2 axis.

Sun Mar 01 00:00:00 EST 2020

Increased matrix metalloproteinase levels and perineuronal net proteolysis in the HIV-infected brain; relevance to altered neuronal population dynamics.

Experimental neurology

Bozzelli PL,Caccavano A,Avdoshina V,Mocchetti I,Wu JY,Conant K

MA3-16888 was used in Western Blotting to show that levels of PNN-degrading matrix metalloproteinases (MMPs) are elevated in HIV-infected post-mortem human brain tissue.

Wed Jan 01 00:00:00 EST 2020

Notch Signaling Regulates MMP-13 Expression via Runx2 in Chondrocytes.

Scientific reports

Xiao D,Bi R,Liu X,Mei J,Jiang N,Zhu S

MA3-16888 was used in Western Blotting to study Notch signalling as a regulator for rat chondrocyte proliferation and diferentiation.

Wed Oct 30 00:00:00 EDT 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Flow Cytometry

Thu Aug 15 00:00:00 EDT 2019

Exosites in Hypervariable Loops of ADAMTS Spacer Domains control Substrate Recognition and Proteolysis.

Scientific reports

Santamaria S,Yamamoto K,Teraz-Orosz A,Koch C,Apte SS,de Groot R,Lane DA,Ahnström J

MA3-16888 was used in Western Blotting to study the role of spacer domain hypervariable loops in the regulation of ADAMTS proteolytic activity.

Mon Jul 29 00:00:00 EDT 2019

Non-pathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Schulz A,Brendler J,Blaschuk O,Landgraf K,Krueger M,Ricken AM

MA3-16888 was used in Immunohistochemistry to investigate the microarchitecture and differentiation of cardiac valve interstitial cells in the transition from juvenile (35 days) to end of adult breeding (2.5 years) stages in zebrafish (ZF, Danio rerio).

Wed May 01 00:00:00 EDT 2019

Intraarticular senescent chondrocytes impair the cartilage regeneration capacity of mesenchymal stem cells.

Stem cell research & therapy

Cao X,Luo P,Huang J,Liang C,He J,Wang Z,Shan D,Peng C,Wu S

MA3-16888 was used in Western Blotting to study the influence of senescent chondrocytes on exogenously transplanted stem cells.

Tue Mar 12 00:00:00 EDT 2019

TLR2 Promotes Vascular Smooth Muscle Cell Chondrogenic Differentiation and Consequent Calcification via the Concerted Actions of Osteoprotegerin Suppression and IL-6-Mediated RANKL Induction.

Arteriosclerosis, thrombosis, and vascular biology

Lee GL,Yeh CC,Wu JY,Lin HC,Wang YF,Kuo YY,Hsieh YT,Hsu YJ,Kuo CC

MA3-16888 was used in Immunohistochemistry to study the role of Toll-like receptor 2 in vascular calcification during atherosclerosis.

Fri Mar 01 00:00:00 EST 2019

Andrographolide mitigates IL‑1β‑induced human nucleus pulposus cells degeneration through the TLR4/MyD88/NF‑κB signaling pathway.

Molecular medicine reports

Zhang L,Chen Q,Wang H,Yang J,Sheng S

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2018

Andrographolide mitigates IL‑1β‑induced human nucleus pulposus cells degeneration through the TLR4/MyD88/NF‑κB signaling pathway.

Molecular medicine reports

Zhang L,Chen Q,Wang H,Yang J,Sheng S

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2018

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Cell death & disease

Li X,Ma R,Gu Q,Liang L,Wang L,Zhang Y,Wang X,Liu X,Li Z,Fang J,Wu J,Wang Y,Li W,Hu B,Wang L,Zhou Q,Hao J

MA3-16888 was used in Immunocytochemistry to characterise a static suspension culture system for large-scale human embryonic stem cell production.

Thu Aug 30 00:00:00 EDT 2018

Three-Dimensional Coculture of Meniscal Cells and Mesenchymal Stem Cells in Collagen Type I Hydrogel on a Small Intestinal Matrix-A Pilot Study Toward Equine Meniscus Tissue Engineering.

Tissue engineering. Part A

Kremer A,Ribitsch I,Reboredo J,Dürr J,Egerbacher M,Jenner F,Walles H

MA316888 was used in immunohistochemistry to compare 3D co-culture constructs of primary equine mesenchymal stem cells and meniscus cells for their qualification to be applied for meniscus tissue engineering

Mon May 01 00:00:00 EDT 2017

Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption.

Scientific reports

Mével E,Merceron C,Vinatier C,Krisa S,Richard T,Masson M,Lesoeur J,Hivernaud V,Gauthier O,Abadie J,Nourissat G,Houard X,Wittrant Y,Urban N,Beck L,Guicheux J

MA3-16888 was used in Immunohistochemistry to conclude that HT/PCy extract may be of clinical significance for the preventive treatment of osteoarthritis.

Mon Sep 19 00:00:00 EDT 2016

Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration.

Gene expression patterns : GEP

Govindan J,Iovine MK

MA3-16888 was used in immunohistochemistry - frozen section to analyze extracellular matrix protein expression during caudal fin regeneration in zebrafish.

Thu Sep 08 00:00:00 EDT 2016

Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration.

PloS one

Govindan J,Tun KM,Iovine MK

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunofluorescence

Tue Jul 19 00:00:00 EDT 2016

Approaching the compressive modulus of articular cartilage with a decellularized cartilage-based hydrogel.

Acta biomaterialia

Beck EC,Barragan M,Tadros MH,Gehrke SH,Detamore MS

MA3-16888 was used in immunohistochemistry - paraffin section to utilize a decellularized cartilage-based hydrogel to help with the compressive modulus of articular cartilage

Fri Jul 01 00:00:00 EDT 2016

Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage.

Annals of biomedical engineering

Beck EC,Barragan M,Tadros MH,Kiyotake EA,Acosta FM,Kieweg SL,Detamore MS

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunohistochemistry

Wed Jun 01 00:00:00 EDT 2016

Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes.

Tissue engineering. Part A

Beck EC,Barragan M,Libeer TB,Kieweg SL,Converse GL,Hopkins RA,Berkland CJ,Detamore MS

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2016

Proteomic profiling and functional characterization of early and late shoulder osteoarthritis.

Arthritis research & therapy

Wanner J,Subbaiah R,Skomorovska-Prokvolit Y,Shishani Y,Boilard E,Mohan S,Gillespie R,Miyagi M,Gobezie R

MA3-16888 was used in western blot to establish comparative proteomic profiles of healthy shoulders and those in the early and late stages of osteoarthritis

Wed Nov 06 00:00:00 EST 2013

Lyme disease spirochaetes possess an aggrecan-binding protease with aggrecanase activity.

Molecular microbiology

Russell TM,Johnson BJ

MA3-16888 was used in western blot to study a novel aggrecanase produced by the Lyme disease spirochaete during human infection and the pathogenic significance

Tue Oct 01 00:00:00 EDT 2013

Effects of a glycogen synthase kinase-3β inhibitor (LiCl) on c-myc protein in intervertebral disc cells.

Journal of cellular biochemistry

Hiyama A,Sakai D,Arai F,Nakajima D,Yokoyama K,Mochida J

Published figure using Aggrecan monoclonal antibody (Product # MA3-16888) in Immunocytochemistry

Sat Oct 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan core protein; Aggrecan core protein 2; aggrecan epidermal growth factor-like domain-1; aggrecan interglobular domain; aggrecan, structural proteoglycan of cartilage; cartilage aggregating proteoglycan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; high density cartilage proteoglycan; large aggregating proteoglycan; large aggregating proteoglycan, antigen

Gene Aliases: ACAN; Agc; AGC1; AGCAN; CSPCP; CSPG1; CSPGCP; MSK16; SEDK

UniProt ID: (Bovine) P13608, (Human) P16112, (Rat) P07897

Entrez Gene ID: (Dog) 403828, (Rabbit) 100009079, (Pig) 397255, (Horse) 100033876, (Bovine) 280985, (Human) 176, (Sheep) 443001, (Guinea pig) 100731672, (Rat) 58968

Molecular Function: extracellular matrix glycoprotein