|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant rat agrin, C-terminal construct.|
|Storage buffer||PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Radioimmune Assays (RIA)||1 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25500 detects all Agrin isoforms from mouse and rat samples. This antibody detects all N1 isoforms, but fails to detect the N4 protein. Also, the antibody will detect the X, Y, and Z splice variants.
MA1-25500 has been successfully used in immunohistochemistry, immunocytochemistry, radioimmunoassay, and Western blot procedures.
The MA1-25500 immunogen is recombinant rat agrin, C-terminal construct. The epitope is located near the first EGF-like repeat.
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.