Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
MA1-25500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AGR 131
Immunogen
Recombinant rat agrin, C-terminal construct.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2273821
Product Specific Information
This antibody detects all N1 isoforms, but fails to detect the N4 protein. Also, the antibody will detect the X, Y, and Z splice variants.
Target Information
Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated "y" and "z" display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Agrin; RP23-139J18.2
Gene Aliases: AGR; Agrin; Agrn; nmf380
UniProt ID: (Rat) P25304
Entrez Gene ID: (Mouse) 11603, (Rat) 25592
dystroglycan binding
calcium ion binding
protein binding
acetylcholine receptor regulator activity
chondroitin sulfate binding
BMP binding
ATPase inhibitor activity
heparan sulfate proteoglycan binding
ion channel binding
transforming growth factor beta binding
sialic acid binding
protease inhibitor
regulation of protein phosphorylation
positive regulation of protein phosphorylation
plasma membrane organization
chemical synaptic transmission
multicellular organism development
synapse assembly
neuromuscular junction development
regulation of synaptic growth at neuromuscular junction
regulation of receptor activity
cell differentiation
positive regulation of protein binding
regulation of GTPase activity
receptor clustering
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
neurotransmitter receptor metabolic process
positive regulation of synaptic growth at neuromuscular junction
positive regulation of transcription from RNA polymerase II promoter
protein heterotetramerization
positive regulation of filopodium assembly
regulation of cardiac muscle contraction
positive regulation of protein tyrosine kinase activity
regulation of microtubule cytoskeleton organization
skeletal muscle acetylcholine-gated channel clustering
regulation of cardiac muscle cell membrane potential
regulation of axon guidance
negative regulation of sodium ion export from cell
negative regulation of sodium:potassium-exchanging ATPase activity
positive regulation of protein geranylgeranylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support