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Invitrogen
Aiolos Monoclonal Antibody (8B2), PE, eBioscience™
FIGURE: 1 / 2
Aiolos Antibody (12-5789-80) in Flow
C57Bl/6 splenocytes were stained with Anti-Mouse CD19 FITC (Product # 11-0193-82) followed by intracellular staining with 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Mouse Aiolos PE (right) using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.
Product Details
12-5789-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
8B2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10733269
Product Specific Information
Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.
Applications Reported: This 8B2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 8B2 antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ikaros family zinc finger protein 3; Zinc finger protein Aiolos; zinc finger protein, subfamily 1A, 3 (Aiolos)
Gene Aliases: 5830411O07Rik; Aiolos; Ikzf3; Zfpn1a3; Znfn1a3
UniProt ID: (Mouse) O08900
Entrez Gene ID: (Mouse) 22780
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
protein binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
C2H2 zinc finger transcription factor
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
regulation of B cell proliferation
B cell activation
regulation of apoptotic process
regulation of B cell differentiation
regulation of lymphocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
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