Invitrogen

Alcohol Dehydrogenase 1B Monoclonal Antibody (OTI4F12)

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Alcohol Dehydrogenase 1B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ADH1B in COS7 cells. Cells were transfected with a plasmid overexpressing ADH1B and probed with a ADH1B monoclonal antibody (Product # MA5-25524).

Alcohol Dehydrogenase 1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ADH1B monoclonal antibody (Product # MA5-25524).

Alcohol Dehydrogenase 1B Antibody in Western Blot (WB) — Western blot analysis of ADH1B in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with ADH1B (Product # MA5-25524) monoclonal antibody.

Product Details

MA5-25524

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Dog, Human, Rat
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI4F12
Immunogen	Full length human recombinant protein of ADH1B produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.89 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2722833

Target Information

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADH, beta subunit; Alcohol dehydrogenase 1B; alcohol dehydrogenase 2 (class I), beta polypeptide; Alcohol dehydrogenase subunit beta; aldehyde reductase; All-trans-retinol dehydrogenase [NAD(+)] ADH1B; epididymis secretory protein Li 117

Gene Aliases: ADH1B; ADH2; HEL-S-117

UniProt ID: (Human) P00325

Entrez Gene ID: (Human) 125

Molecular Function: dehydrogenase oxidoreductase metabolite interconversion enzyme