Invitrogen

Alcohol Dehydrogenase 1B Monoclonal Antibody (OTI4F12)

Product Image Gallery

Alcohol Dehydrogenase 1B Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of ADH1B in COS7 cells. Cells were transfected with a plasmid overexpressing ADH1B and probed with a ADH1B monoclonal antibody (Product # MA5-25524).

Alcohol Dehydrogenase 1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ADH1B monoclonal antibody (Product # MA5-25524).

Alcohol Dehydrogenase 1B Antibody in Western Blot (WB) — Western blot analysis of ADH1B in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with ADH1B (Product # MA5-25524) monoclonal antibody.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:500

Product Specifications

Species

Dog, Human, Rat

Expression System

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI4F12

Immunogen

Full length human recombinant protein of ADH1B produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

0.89 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2722833

Target Information

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acetaldehyde dehydrogenase 5; ADH, beta subunit; Alcohol dehydrogenase 1B; alcohol dehydrogenase 2 (class I), beta polypeptide; Alcohol dehydrogenase subunit beta; aldehyde dehydrogenase 1 family, member B1; aldehyde dehydrogenase 1B1; Aldehyde dehydrogenase 5; Aldehyde dehydrogenase family 1 member B1; Aldehyde dehydrogenase X, mitochondrial; aldehyde reductase; ALDH class 2; All-trans-retinol dehydrogenase [NAD(+)] ADH1B; epididymis secretory protein Li 117

Gene Aliases: ADH1B; ADH2; ALDH1B1; ALDH5; ALDHX; HEL-S-117

UniProt ID: (Human) P00325, (Human) P30837, (Rat) Q66HF8

Entrez Gene ID: (Human) 125, (Dog) 481620, (Human) 219, (Rat) 298079

Molecular Function: dehydrogenase oxidoreductase reductase