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FIGURE: 1 / 1
Alcohol Dehydrogenase Antibody (200-4143-0100) in WB
Western Blot of Alcohol Dehydrogenase Antibody. Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 Blocking Buffer for 30 min at RT. Predicted: 37kDa/Observed size: 45kDa.
Product Details
200-4143-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Alcohol Dehydrogenase [Yeast]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.^M
^M
Anti-ALCOHOL DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.
Target Information
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADHI; Alcohol dehydrogenase 1; Alcohol dehydrogenase I; NADH-dependent methylglyoxal reductase; YADH-1
Gene Aliases: ADC1; ADH1; O0947; YOL086C
UniProt ID: (Baker's yeast) P00330
Entrez Gene ID: (Baker's yeast) 854068
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