Invitrogen

Aldolase C Polyclonal Antibody

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Datasheet

Protocols

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Aldolase C Antibody in Western Blot (WB) — Western Blot analysis of Aldolase C using Aldolase C Polyclonal Antibody (Product # PA5-112299) at a dilution of 1:500. Lane A: U87-MG whole cell lysate, Lane B: MCF-7 whole cell lysate, Lane C: Jurkat whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 39 kDa.

Aldolase C Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Aldolase C was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of Aldolase C Polyclonal Antibody (Product # PA5-112299) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 39 kDa.

Product Details

PA5-112299

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunoprecipitation (IP)	1-5 µL/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human Aldolase C/ALDOC fragment
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2867035

Target Information

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aldolase 3; aldolase C, fructose-bisphosphate; Brain-type aldolase; EC 4.1.2.13; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase; Fructose-bisphosphate aldolase C

Gene Aliases: ALDC; ALDOC

UniProt ID: (Human) P09972

Entrez Gene ID: (Human) 230

Molecular Function: aldolase

Function(s)

fructose metabolic process gluconeogenesis fructose 1,6-bisphosphate metabolic process epithelial cell differentiation canonical glycolysis

Process(es)

fructose-bisphosphate aldolase activity protein binding cytoskeletal protein binding

Aldolase C Polyclonal Antibody

Product Details

PA5-112299

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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