Fixed and permeabilized HeLa cells were stained with MMP16/sRAGE ABfinity™ Recombinant Rabbit oligoclonal Antibody, followed by Alexa Fluor 488 goat anti Rabbit Ig (right peak). Quenching was performed on these labelled cells using Alexa Fluor 488 ABfinity™ Recombinant Rabbit oligoclonal Antibody (middle peak). Isotype control (left peak).
|Tested species reactivity||Chemical|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alexa Fluor® 488 dye conjugated to KLH|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Fluorescent Quenching (FQ)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors. The ABfinity™ Alexa Fluor® 488 Recombinant Rabbit Oligoclonal Antibody has a high affinity for the Alexa Fluor® 488 fluorophore, and quenches most of the fluorescence of the dye upon binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.