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Invitrogen
Alix Monoclonal Antibody (3A9)
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FIGURE: 1 / 34
Alix Antibody (MA1-83977) in ICC/IF
Immunofluorescence analysis of Alix was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Alix Monoclonal Antibody (3A9) (Product # MA1-83977) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1... View More
Product Details
MA1-83977
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Xenopus
Published species
Bacteria,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3A9
Immunogen
Alix fusion protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product does not require protein digestion pre-treatment of paraffin sections. A ~100kD band is detected by Western blot.
Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95.
Target Information
Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein 1; Alg2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; E2F1-inducible protein; Eig2; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein; putative signal tranduction protein Xp95; Signal transduction protein Xp95; similar to XP95; possible role in signal transduction and cell growth/death; unnamed protein product
Gene Aliases: AI480591; AIP1; ALIX; AW544830; C76364; DRIP4; Eig2; HP95; KIAA1375; MCPH29; mKIAA1375; PDCD6IP; RGD1561176; XELAEV_18031317mg; xp95
UniProt ID: (Human) Q8WUM4, (Rat) Q9QZA2, (Mouse) Q9WU78, (Xenopus) Q9W6C5
Entrez Gene ID: (Human) 10015, (Rat) 501083, (Mouse) 18571, (Xenopus) 398095
mitotic cytokinesis
actomyosin contractile ring assembly
apoptotic process
nucleus organization
mitotic metaphase plate congression
regulation of centrosome duplication
protein transport
macroautophagy
multivesicular body assembly
viral budding via host ESCRT complex
maintenance of epithelial cell apical/basal polarity
viral budding
protein homooligomerization
cell division
midbody abscission
bicellular tight junction assembly
regulation of membrane permeability
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
extracellular exosome biogenesis
regulation of mitotic spindle assembly
positive regulation of exosomal secretion
regulation of extracellular exosome assembly
positive regulation of extracellular exosome assembly
cell separation after cytokinesis
transport
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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