Immunohistochemistry analysis of Alpha Fetoprotein showing staining in the cytoplasm of paraffin-embedded human hepatocellular carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an Alpha Fetoprotein monoclonal antibody (Product # MA5-12754) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Raised against affinity purified alpha fetoprotein (AFP) from serum of a hepatoma patient|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12754 targets Alpha Fetoprotein in immunohistochemistry applications and shows reactivity with Human and Mouse samples. Does not react with Bovine, Rat, or Feline samples.
The MA5-12754 immunogen is raised against affinity purified alpha fetoprotein (AFP) from serum of a hepatoma patient.
AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extrarenal teratoid Wilms' tumor: two cases in unusual locations, one associated with elevated serum AFP.
MA5-12754 was used in immunohistochemistry to investigate the clinicopathologic features of extrarenal teratoid Wilms tumor and its alpha fetaprotein expression
|Song JS,Kim IK,Kim YM,Khang SK,Kim KR,Lee Y||Pathology international (60:35)||2010|
Hep Par 1 expression in carcinoma of the cervix: implications for diagnosis and prognosis.
MA5-12754 was used in immunohistochemistry to investigate the usefulness of Hep Par 1 level for cervical cancer diagnosis and prognosis
|Thamboo TP,Wee A||Journal of clinical pathology (57:48)||2004|
Microchip-based multiplex electro-immunosensing system for the detection of cancer biomarkers.
MA5-12754 was used in ELISA to develop a novel multiplex electro-immunosensing microchip system for cancer biomarker detection
|Ko YJ,Maeng JH,Ahn Y,Hwang SY,Cho NG,Lee SH||Electrophoresis (29:3466)||2008|
A novel microfluidic biosensor based on an electrical detection system for alpha-fetoprotein.
MA5-12754 was used in ELISA to develop a novel electrical immunoassay biosensor for alpha-fetoprotein detection
|Maeng JH,Lee BC,Ko YJ,Cho W,Ahn Y,Cho NG,Lee SH,Hwang SY||Biosensors and bioelectronics (23:1319)||2008|