Invitrogen

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience™

Product Image Gallery

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma using 5 µg/mL of Mouse IgG2a K Isotype Control eFluor® 660 (left) or 5 µg/mL of Anti-Alpha-Smooth Muscle Actin eFluor® 660 (right). Nuclei are stained with DAPI.

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF) — Fig. 6 AGEs-RAGE induced EndMT by mediating endothelial autophagy in HUVECs. A Confocal microscopic image of double immunofluorescence staining for CD31 (green) and alpha-SMA (red); nuclei were counter stained with DAPI (blue); (Scale bars = 100 um). B Protein expression of Beclin 1, LC3BII/I, RAGE, Collagen I, N-Cadherin, VE-Cadherin, CD31, and alpha-SMA assessed by western blotting. Quantification of RAGE ( C ), COL I ( D ), N-Cadherin ( E ), VE-Cadherin ( F ), CD31 ( G ), alpha-SMA ( H ), Beclin 1 ( I ), and LC3BII/I ( J ). GAPDH was used as the internal control. n = 6. Data are presented as mean +- SEM. * p < 0.05 vs. control group.

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 2 Treg cell ablation results in advanced tumor staging. (A,B) 10-week-old mammary gland whole mount representative images (A) and quantification (B) of the percentage of tumor area of control (black) and Treg cell-ablated (green) mice ( n = 16-17 individual mammary glands). Scale bars represent 5 mm, * p = 0.0405 calculated by Mann-Whitney test. (C,D) Representative image (C) and quantification (D) of tumor stage. Arrows indicate examples of various tumor stages: hyperplasia/adenoma (green arrow), early invasive carcinoma (black arrow), and late invasive carcinoma (white arrow) (E) alpha-SMA myoepithelial staining confirming disruption of myoepithelial layer (F,G) , representative image (F) and quantification (G) of desmoplasia; (H,I) representative image of Masson trichome staining (H) and quantification (I) of collagen deposition; and (J,K) representative image (J) and quantification (K) of inflammatory infiltration. (E,F,H,J) White arrows indicate examples of histological observation. Values are expressed as mean +- SEM. Tumor staging was compared by two-way ANOVA, **** p < 0.0001; other comparisons were done by Mann-Whitney test. **** p < 0.0001 and ** p = 0.0021. Data were pooled from four independent experiments. (C,E,F,H,J) Scale bars represent 250 mum.

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 3 Cardiac mural cells originate from endothelial cells. ( a - d ) Clonal analysis of Cdh5-CreERT2 Rosa26-mTmG double-transgenic mice. ( a ) Clonal analysis strategy of Cdh5-CreERT2 Rosa26-mTmG mice indicating the stages of 4-hydroxytamoxifen (4-OHT) administration and analysis. ( b ) Overview of GFP+ cell distribution in heart at E16.5. ( c ) Images showing widespread distribution of EC-derived GFP+ PDGFRbeta+ mural cells (arrows). Arrowheads mark GFP- PDGFRbeta+ cells. ( d ) Higher magnification images showing vessel-associated (isolectin B4), EC-derived GFP+ PDGFRbeta+ NG2+ mural cells (arrows) at E16.5 (top and middle row) and GFP+ alphaSMA+ mural cells (arrows) at E18.5 (bottom row). Arrowheads mark GFP+ (green) isolectin B4+ (blue) ECs. Grey channel was not included in merged image. ( e ) Lineage tracing in Tie2-Cre Rosa26-mTmG double-transgenic hearts. Confocal images showing GFP+ PDGFRbeta+ NG2+ and GFP+ alphaSMA+ mural cells (arrows) in association with myocardial capillary ECs (isolectin B4). Arrowheads mark GFP+ (green) isolectin B4+ (blue) ECs. Grey channel was not included in merged image.

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC) — FIGURE 7 Immunohistochemical staining for alpha-smooth muscle actin in lung sections (A) An image from the vehicle control group (group I) shows mild cytoplasmic expression of smooth muscle actin antibody in perivascular and peribronchiolar muscle coat with also expression in scattered few mesenchymal cells in interalveolar septa. Compared to its gradually and progressively increased expression in leflunomide 2.5 group (B) , leflunomide 5 group (C) and leflunomide 10 group (D) (E) A column chart representing the mean area +- SDM, (A) : vs. the vehicle group, (B) : vs. the leflunomide 2.5 mg per kg group, (C) : vs. the leflunomide 5 mg per kg group at p < 0.05.

Alpha-Smooth Muscle Actin Antibody in Western Blot (WB) — Fig. 5 Intercellular transfer of miR-133 by H/R-induced EPC-derived exosomes inhibits fibroblast MEndoT. a Endothelial markers CD31, VE-cadherin, and vWF and fibrosis markers alpha-SMA, N-cadherin, vimentin, and collagen I were measured by western blotting in fibroblasts treated with normal cultured EPC-derived exosomes, H/R-induced EPC-derived exosomes, H/R+NC inhibitor-induced EPC-derived exosomes, and H/R+miR-133 inhibitor-induced EPC-derived exosomes. b - h The bar graph represents quantification of endothelial markers CD31 ( b ), VE-cadherin ( c ), and Vwf ( d ) and fibrosis markers alpha-SMA ( e ), N-cadherin ( f ), vimentin ( g ), and collagen I ( h ) expression per group. *** P < 0.001, &&& P < 0.001

Alpha-Smooth Muscle Actin Antibody in Flow Cytometry (Flow) — Fig. 4 EndMT is one origin of myofibroblasts in HF and could be regulated by AGEs/RAGE and autophagy. A Flow cytometry analysis of primary isolates of mouse left ventricular cells. After mechanical disaggregation and enzymatic digestion, single-cell suspensions were incubated with combination of fluorescent antibodies to CD31, VE-Cadherin, and alpha-SMA. B , C Quantification of co-expressed cell analysis. n = 5. Data are presented as mean +- SEM. * p < 0.05 vs. sham group, # p < 0.05 vs. TAC group.

Product Details

50-9760-80

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	5 µg/mL	-
Immunohistochemistry (Frozen) (IHC (F))	5 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	View 7 publications 7 publications
Flow Cytometry (Flow)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	1A4
Conjugate	eFluor™ 660 Alexa Fluor 488 eFluor 570 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574361

Product Specific Information

Description: The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin. Alpha-smooth muscle actin is a 42 kDa protein and is a major component of the cytoskeletal structural network. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. It is also expressed in the parenchyma and stroma of various tissues. Alpha-smooth muscle actin is useful in the identification of leiomyomas, leiomycosarcomas and pleomorphic adenomas and is used as a prognostic marker for basal cell carcinoma.

Applications Reported: This 1A4 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 1A4 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 1A4 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production.

Frontiers in pharmacology

El-Sherbiny M,Atef H,Eladl MA,Mohamed AS,El-Shafey M,Ali HS,Zaitone SA,Alomar SY,Alqahtani SAM,Aloyouni SY,Attia MA

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity.

Nature immunology

Prados A,Onder L,Cheng HW,Mörbe U,Lütge M,Gil-Cruz C,Perez-Shibayama C,Koliaraki V,Ludewig B,Kollias G

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Gut-liver axis modulation of Panax notoginseng saponins in nonalcoholic fatty liver disease.

Hepatology international

Xu Y,Wang N,Tan HY,Li S,Zhang C,Feng Y

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus.

Cancer biology & therapy

Wang T,Xie S,Luo R,Shi L,Bai P,Wang X,Wan R,Deng J,Wu Z,Li W,Xiao W,Wang Y,Chen B,Zhang K,Xing J

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Mon Mar 29 00:00:00 EDT 2021

Maintaining resting cardiac fibroblasts in vitro by disrupting mechanotransduction.

PloS one

Gilles G,McCulloch AD,Brakebusch CH,Herum KM

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Dec 21 00:00:00 EST 2020

Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis.

JCI insight

Lu S,Jolly AJ,Strand KA,Dubner AM,Mutryn MF,Moulton KS,Nemenoff RA,Majesky MW,Weiser-Evans MC

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Thu Dec 03 00:00:00 EST 2020

Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis.

JCI insight

Lu S,Jolly AJ,Strand KA,Dubner AM,Mutryn MF,Moulton KS,Nemenoff RA,Majesky MW,Weiser-Evans MC

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Thu Dec 03 00:00:00 EST 2020

Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.

Journal of thrombosis and haemostasis : JTH

Suess PM,Smith SA,Morrissey JH

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Sun Nov 01 00:00:00 EDT 2020

The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity.

Scientific reports

Gorchs L,Ahmed S,Mayer C,Knauf A,Fernández Moro C,Svensson M,Heuchel R,Rangelova E,Bergman P,Kaipe H

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Thu Oct 15 00:00:00 EDT 2020

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Tue Sep 29 00:00:00 EDT 2020

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Tue Sep 29 00:00:00 EDT 2020

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro.

International journal of cell biology

Polley A,Khanam R,Sengupta A,Chakraborty S

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Physiologically Relevant Fluid-Induced Oscillatory Shear Stress Stimulation of Mesenchymal Stem Cells Enhances the Engineered Valve Matrix Phenotype.

Frontiers in cardiovascular medicine

Gonzalez BA,Perez-Nevarez M,Mirza A,Perez MG,Lin YM,Hsu CD,Caobi A,Raymond A,Gomez Hernandez ME,Fernandez-Lima F,George F,Ramaswamy S

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula.

Journal of vascular research

Somarathna M,Isayeva-Waldrop T,Al-Balas A,Guo L,Lee T

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Mon Sep 14 00:00:00 EDT 2020

Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene-Deficient Mouse Model of Pancreatic Cancer.

Translational oncology

Yazawa K,Nakamura F,Masukawa D,Sato S,Hiroshima Y,Yabushita Y,Mori R,Matsuyama R,Kato I,Taniguchi H,Goshima Y,Endo I

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2020

Material-Induced Venosome-Supported Bone Tubes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Charbonnier B,Baradaran A,Sato D,Alghamdi O,Zhang Z,Zhang YL,Gbureck U,Gilardino M,Harvey E,Makhoul N,Barralet J

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Wed Sep 04 00:00:00 EDT 2019

YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.

Stem cell research & therapy

Lin F,Zeng Z,Song Y,Li L,Wu Z,Zhang X,Li Z,Ke X,Hu X

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Western Blot

Fri Aug 23 00:00:00 EDT 2019

Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.

Nature communications

Diéguez-Hurtado R,Kato K,Giaimo BD,Nieminen-Kelhä M,Arf H,Ferrante F,Bartkuhn M,Zimmermann T,Bixel MG,Eilken HM,Adams S,Borggrefe T,Vajkoczy P,Adams RH

50-9760-82 was used in Immunohistochemistry to propose that brain pericytes can acquire deleterious properties that actively enhance vascular lesion formation and promote pathogenic processes.

Thu Jun 27 00:00:00 EDT 2019

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunofluorescence

Wed Jul 11 00:00:00 EDT 2018

Human ISL1⁺ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction.

Molecular therapy : the journal of the American Society of Gene Therapy

Foo KS,Lehtinen ML,Leung CY,Lian X,Xu J,Keung W,Geng L,Kolstad TRS,Thams S,Wong AO,Wong N,Bylund K,Zhou C,He X,Jin SB,Clarke J,Lendahl U,Li RA,Louch WE,Chien KR

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Thu Jul 05 00:00:00 EDT 2018

Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system.

Nature communications

Kaur H,Carvalho J,Looso M,Singh P,Chennupati R,Preussner J,Günther S,Albarrán-Juárez J,Tischner D,Classen S,Offermanns S,Wettschureck N

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Fri Jun 16 00:00:00 EDT 2017

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunofluorescence

Fri Aug 12 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; Alpha Cardiac Actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA

UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737

Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475

Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein