Invitrogen

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor 660, eBioscience™

Product Image Gallery

Alpha-Smooth Muscle Actin Antibody in Immunofluorescence (IF) — Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma using 5 µg/mL of Mouse IgG2a K Isotype Control eFluor® 660 (left) or 5 µg/mL of Anti-Alpha-Smooth Muscle Actin eFluor® 660 (right). Nuclei are stained with DAPI.

Alpha-Smooth Muscle Actin Antibody — Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-80)

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

5 µg/mL

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Published Applications

Flow Cytometry (Flow)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host / Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1A4

Conjugate

eFluor® 660

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2574361

Product Specific Information

Description: The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin. Alpha-smooth muscle actin is a 42 kDa protein and is a major component of the cytoskeletal structural network. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. It is also expressed in the parenchyma and stroma of various tissues. Alpha-smooth muscle actin is useful in the identification of leiomyomas, leiomycosarcomas and pleomorphic adenomas and is used as a prognostic marker for basal cell carcinoma.

Applications Reported: This 1A4 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 1A4 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 1A4 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha-actin-2; alpha-cardiac actin; Cell growth-inhibiting gene 46 protein; smooth muscle alpha-actin

Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA

UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737

Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475

Molecular Function: actin and actin related protein actin family cytoskeletal protein cytoskeletal protein