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Immunofluorescent analysis of Alpha methylacyl-CoA-racemase (green) showing staining in the in the peroxisomes and mitochondria of human prostate carcinoma tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with an Alpha methylacyl-CoA-racemase monoclonal antibody (Product # MA5-14576) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human p504s/AMACR polypeptide|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14576 targets Alpha methylacyl-CoA-racemase in WB, IF and IHC (P) applications and shows reactivity with Human samples.
The MA5-14576 immunogen is human p504s/AMACR polypeptide.
Alpha methylacyl-CoA-racemase (AMACR), also known as p504s is an enzyme involved in the metabolism of branched-chain fatty acids. p504s is expressed in various normal tissues and carcinomas, while it is overexpressed over 30-fold in prostate carcinoma compared to benign glandular tissues1. When p504s is overexpressed in atypical glands without basal cells, it establishes a diagnosis of prostatic carcinoma. High-grade prostatic intraepithelial neoplasia, atypical adenomatous hyperplasia and urothelial carcinoma may also express p504s.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AMACR; AMACRD; CBAS4; RACE; RM