Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human small intestine stained using a Ambra1 polyclonal antibody (Product # PA5-23212) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Bovine, Chicken, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (24 GAQRLLQELVEDKTR 38) of Ambra1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Ambra1, a WD40 domain containing protein, is a novel activating molecule in Beclin 1-regulated autophagy and plays a role in the development of the nervous system. The protein binds to Beclin 1 and favors Beclin 1/Vps34 interaction. The Ambra1 gene localized on human chromosome 11p11.2, encodes the protein that is highly conserved in vertebrates only and its expression is mostly confined to the neuroepithelium during early neurogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.